ATP Content: An Indicator of Sperm Metabolic Fitness

ATP Content: An Indicator of Sperm Metabolic Fitness

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Author Info

Corresponding Author
Rajasingam S. Jeyendran
Androlab Inc, Chicago, Illinois, USA


The association of sperm adenosine triphosphate (ATP) content with the standard sperm parameters and among different populations of sperm within an ejaculate was determined. ATP was determined in sperm using Promega CellTiter-GloR 2.0 and the chemiluminescence read on a Molecular Devices SpectraMax L plate reader. ATP was calculated per 106 sperm and all results were presented as mean ± standard deviation. Pearson correlation coefficients between ATP content per 106 sperm and all other variables analysed had no significant association (p <0.05). Student t-test however yielded significant differences among motile and progressively motile sperm in the filtrate and retentate respectively, following silica wool filtration (p<0.0005). This rapid, quantitative ATP assay provides new information regarding overall metabolic function of the sperm independent of other sperm variables, especially sperm motility. Future studies of pregnancy outcome will corroborate whether this assay helps to identify the minimum number of metabolically active sperm needed to classify an ejaculate as fertile, sub-fertile or infertile.

Article Info

Article Type
Research Article
Publication history
Received: Fri 10, Jun 2022
Accepted: Wed 29, Jun 2022
Published: Wed 13, Jul 2022
© 2023 Rajasingam S. Jeyendran. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.CROGR.2022.01.02