STAT3 Knockdown Induces Tumor Formation by MDA-MB-231 Cells

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Author Info

Cindy Pru Haluk Resat James K. Pru Kasturi Banerjee

Corresponding Author

Kasturi Banerjee
The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman,WA,USA

A B S T R A C T

STAT3 plays a central role in oncogenesis by mediating cell survival, growth, and differentiation. It is constitutively activated in breast cancer. We investigated the role of STAT3 in tumor development by knocking down STAT3 levels in MDA-MB-231 triple negative breast cancer cells using short hairpin RNA. The tumor forming potential of these STAT3-depleted cells was assessed by xenografts in immunocompromised NOD SCID mice. Contrary to its accepted tumor promoting role, we found STAT3 to be a negative regulator of growth in MDA-MB-231- derived tumors. Although similar observations have been made in thyroid carcinoma and lung adenocarcinoma xenograft studies, our novel results showed for the first time that the role of STAT3 in promoting tumorigenesis may be context-specific, and that STAT3 may actually be a negative regulator of certain breast-cancer types. Studies to identify the mechanisms of STAT3’s negative regulatory role may be useful in developing STAT3-based therapeutics. Abbreviations: Breast cancer, BC; triple negative breast cancer, TNBC; signal transducer and activator of transcription, STAT.

Article Info

Article Type

Research Article

Publication history

Received: Mon 26, Feb 2018
Accepted: Wed 07, Mar 2018
Published: Mon 26, Mar 2018

Copyright

© 2023 Kasturi Banerjee. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.COR.2018.10.002