Inhibitory effect of quercetin on protein expression CtBP1 and migration of hepatocellular carcinoma cell SMMC-7721

Author Info

Jianhong Tang Lihui Bian Shengjun Xiao Xiaoling Zhang Xiaoyu Wang Yueli Yang

Corresponding Author

Xiaoling Zhang
Department of Physiology, Faculty of Basic Medical Sciences, Guilin Medical University, Guilin, Guangxi 541004, People's Republic of China

A B S T R A C T

Our study aimed to investigate the potential role of quercetin in the migration of hepatocellular carcinoma cells, SMMC-7721, and its underlying mechanism. Cell monolayer scratching assay was performed to explore the role of quercetin in the migration ability of SMMC-7721 cells. Western Blot and immunofluorescence assay were conducted to detect the protein expression of transcriptional co-suppressor, CtBP1, and epithelial adhesion molecule, E-cadherin. The migration ability of SMMC-7721 cells was decreased by the addition of quercetin in a time and concentration dependent manner. Results of western blot and immunofluorescence assay demonstrated that quercetin inhibited the expression of CtBP1, while up-regulated the expression of E-cadherin. These findings suggest that quercetin contributed to the upregulation of E-cadherin through inhibition of CtBP1 expression. This in turn hinders the underlying EMT process, and finally prevents the invasiveness and metastasis of HCC cells. However, the mechanism how quercetin up-regulation CtBP1 in HCC cells still need to be elucidated.

Article Info

Article Type

Research Article

Publication history

Received: Fri 25, May 2018
Accepted: Mon 04, Jun 2018
Published: Mon 11, Jun 2018

Copyright

© 2023 Xiaoling Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.COR.2018.02.001