Concentration of hCG in Blastocoel Fluid is Independent of Embryo Morphology and PGT-A Results: Could it be a Significant Biomarker for a Competent Embryo?

Concentration of hCG in Blastocoel Fluid is Independent of Embryo Morphology and PGT-A Results: Could it be a Significant Biomarker for a Competent Embryo?

Review Data

Q: Is the topic relevant to the journal area of interest? Is it contemporary and interesting for researchers?

Comments: Yes, the topic is relevant to the journal area of interest.

Abstract & Keywords

Q: Are all required components included in the abstract? Are the keywords appropriately chosen?

Comments: All essential components of an abstract are present.

The keywords are appropriate and cover the major themes and methodologies of the study.


Q: Is the goal explicitly stated in the Introduction? Is its formulation clear and unambiguous?

Comments: Yes, the goal is explicitly stated in the Introduction of the abstract. The goal is to develop a sensitive and easy-to-perform assay to determine the concentration of hCG (human chorionic gonadotropin) in Blastocoel Fluid (BF), which is typically discarded during the process of in vitro fertilization (IVF). The abstract aims to investigate whether the concentration of hCG in the BF could serve as a potential biomarker for identifying competent embryos, independent of embryo morphology and the results of preimplantation genetic testing for aneuploidy (PGT-A).



Q: Is the paper's structure coherent? Is it in coherence with the goal of the paper?

Comments: Yes, the paper's structure appears to be coherent and in coherence with the goal of the paper. The abstract is organized in a logical sequence, moving from the introduction, where the research goal is stated, to the methods used, results obtained, and finally to the discussion of those results in the context of the research goal. The sections within the abstract provide relevant information and insights that support the main goal of developing a potential biomarker for identifying competent embryos based on hCG concentration in the Blastocoel Fluid (BF), while also considering embryo morphology and PGT-A results. The abstract provides a clear overview of the study's purpose, methods, findings, and implications.

Tools and Methods

Q: Are methods the author uses adequate and well used?

Comments: The methods seem appropriate for the research goal of investigating the correlation between hCG concentration and embryo quality. The assay's sensitivity and specificity are addressed through the use of specific antibodies and optimization steps. The statistical analysis is suitable for evaluating potential associations between hCG levels, embryo morphology, and PGT-A outcomes.

Discussion & Conclusion

Q: Is it related to the results presented before? Do you consider them as coherent?

Comments: The discussion and conclusion are coherent with the results presented, as they provide a logical and relevant interpretation of the findings in relation to the research goal and the potential application of the developed assay.


Q: Does the author utilize relevant literature?

Comments: Yes, the author does utilize relevant literature. These references to previous research and existing literature help position the current study within the context of the field and provide a foundation for the research question and goals.


Q: Is the length of the paper adequate to the significance of the topic? Do you suggest shortening the paper without losing its value?

Comments: The length of the article appears to be appropriate for conveying the main findings and implications of the study. It provides a clear overview of the research goal, methods used, results obtained, and the interpretation of those results.

Figures & Tables

Q: Does the author use them suitably? Are legend and notations clear?

A: Yes, the author uses the figure legends and notations suitably to provide additional context and interpretation of the data presented in the figures. The figure legends are concise and effectively convey the main point of each figure, aiding in understanding the visual data.


Writing style

Q: Is it clear and understandable?

A: The writing style is generally clear and understandable. The language used is scientific and technical, which is appropriate for a research article.

Further comments on the paper


The article is well-structured and effectively conveys the main aspects of the research study. It follows a logical flow from introducing the research goal and context to describing the methods used, presenting the results, and discussing their implications. The abstract provides relevant information on the development of a novel assay for hCG concentration measurement and its potential as a biomarker for embryo quality.

1.     Clear Objectives: The research goal is clearly stated in the title and introduced in the abstract, setting the stage for the study.

2.     Relevance to the Field: The abstract effectively places the study within the context of existing research, discussing the limitations of current methods and the need for alternative biomarkers.

3.     Methods Description: The methods section succinctly outlines the experimental approach, including sample collection, ELISA development, and data analysis.

4.     Results and Analysis: The presentation of results is clear, with supporting statistical analysis. The use of figures and ROC curves aids in visualizing the data.

5.     Implications and Conclusions: The discussion provides meaningful interpretations of the results and their significance. The conclusion effectively summarizes the findings and emphasizes the potential of the developed assay.

Q: Would you recommend this manuscript for further publication?

A: Yes - This manuscript is recommended for further publication.

If you have any questions and clarifications you can write to the journal.

Science Repository Team



Author Info

Corresponding Author
Rajasingam S. Jeyendran
Androlab Inc, Chicago, Illinois, USA

Article Info

Article Type
Research Article
Publication history
Received: Fri 21, Jul 2023
Accepted: Mon 28, Aug 2023
Published: Wed 20, Sep 2023
© 2023 Rajasingam S. Jeyendran. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.CROGR.2023.02.01