T-Cell Receptor Clustering Methods Used for Single Cell Analysis: Potential Application in Oncology

Review Data

Q: Is the topic relevant to the journal area of interest? Is it contemporary and interesting for

researchers?

A: Very good

Abstract & Keywords

Q: Are all required components included in the abstract? Are the keywords appropriately chosen?

A: Very good

Goal

Q: Is the goal explicitly stated in the Introduction? Is its formulation clear and unambiguous?

A: Good

Structure

Q: Is the paper's structure coherent? Is it in coherence with the goal of the paper?

A: Good

Tools and Methods

Q: Are methods the author uses adequate and well used?

A: Good

Discussion & Conclusion

Q: Is it related to the results presented before? Do you consider them as coherent?

A: Very good

Comments:

The Discussion section summarizes from the points of the review that the identification and quantification of receptor clustering provides crucial information about current cellular immune state and activation. It further states that more specific methods for researching the real-time thermally driven adhesion and dissociation between lymphocyte receptor clustering in response to antigen contact can be provided by using Optical Trapping-Optical Tweezers. The study Concludes that the importance of the movement of receptor molecules within the membrane structure is significant in better understanding of the fundamentals of immunity and cancer immunotherapy.

Literature

Q: Does the author utilize relevant literature?

A: Very good

Author's knowledge

Q: What is the level of the author’s knowledge? Does the author utilize all recent contributions relevant to the topic?

A: Good

Length

Q: Is the length of the paper adequate to the significance of the topic? Do you suggest shortening the paper without losing its value?

A: Good

Figures & Tables

Q: Does the author use them suitably? Are legend and notations clear?

A: Very good

Writing style

Q: Is it clear and understandable?

A: Good

Further comments on the paper

Comments: This review article aims to investigate how receptor clustering that has been studied, can be more effectively studied in the future and what the current evidence suggests about T-cell receptor (TCR) clustering/T-cell activity relationships. This review specifically examines TCR/CD3 molecule clustering, and the techniques used to study these single cell activities. There is a need of further research in order to advance the understanding of TCR clustering to facilitate a better understanding of how T-Cell activation occurs.

Q: Would you recommend this manuscript for further publication?

A: Yes - Suitable to be published

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Science Repository Team