Concentration of hCG in Blastocoel Fluid is Independent of Embryo Morphology and PGT-A Results: Could it be a Significant Biomarker for a Competent Embryo?

Author Info

Rajasingam S. Jeyendran Michael Kirk Antony Anderson Milica Ivanovic Seth Levrant Aleksandra Lazarevic Elizabeth Puscheck

Corresponding Author

Rajasingam S. Jeyendran
Androlab Inc, Chicago, Illinois, USA

A B S T R A C T

The aim of this research was to develop a quantitative sandwich-strip ELISA was developed on a nitrocellulose membrane with anti-H alpha and anti-hCG beta antibodies. The detection antibody was conjugated to horseradish peroxidase, and a quantitative sandwich-strip ELISA with luminol substrate was developed following optimization with hCG. Concentration of hCG in the BF was determined for 101 embryo biopsies (Study 1) and 96 embryo biopsies (Study 2) respectively, and the results were compared with the previously recorded embryo morphology and PGT-A results. hCG was present in 73.6% of all BF specimens analysed in these two studies, and a weak correlation between hCG levels and both embryo morphology and PGT-A outcome was observed. Additionally, the receiver operating characteristic (ROC) curves show separately that both embryo morphology and PGT-A do not correlate with hCG concentrations. This indicates that the presence of hCG produced in the BF by the embryo is a separate, independent marker of embryo quality.

Article Info

Article Type

Research Article

Publication history

Received: Fri 21, Jul 2023
Accepted: Mon 28, Aug 2023
Published: Wed 20, Sep 2023

Copyright

© 2023 Rajasingam S. Jeyendran. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.CROGR.2023.02.01