Cytomorphomertic analysis of buccal cells of steel workers exposed to chromium in south indian population

Author Info

D Deepak S Sushmitha Saranya R Singaravelu

Corresponding Author

Saranya R Singaravelu
Assistant Professor, PG & Research Department of Biotechnology, Hindusthan College of Arts and Science, Nava India, Coimbatore, India

A B S T R A C T

Steel workers are exposed to a mixture of chemicals and chromium (IV) and (III), which is suspected to cause genetic damage. And to evaluate the MN assay along with the cellular diameter (CD) and nuclear diameter (ND) in the buccal epithelial cells of workers residing in Coimbatore District, South India, using cytomorphometric analysis. Methods: 40 samples from steel workers and 20 samples were exposed, 20 as standardized control group were examined for frequencies of micronucleus (MN) in buccal epithelial cells. PAP staining techniques were used to examine the nucleus and micronucleus. The frequency of micronuclei is more in smokers group when compared to alcohol consumers. Results: The genetic damage observed in the buccal cells of steel workers was significantly higher than in controls with cigarettes smoking in exposed group (12.28 ± 0.61) and alcohols in exposed group (12.61 ± 0.46) while compare to that of non-smoking and non-alcohols workers (6.94 ± 0.60 and 6.47 ± 0.44). Conclusions: Occupational exposure of chromium from steel workers has been associated with increased genetic damage in smoking and alcohol habits represent an additional risk factor. Exposure of chromium may be related to increased risk of cancer in steel workers.

Article Info

Article Type

Research Article

Publication history

Received: Mon 12, Mar 2018
Accepted: Fri 23, Mar 2018
Published: Mon 02, Apr 2018

Copyright

© 2023 Saranya R Singaravelu. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.COR.2018.10.006