BDE-209 and TCDD Modulate the Expression and Activity of ATP-Binding Cassette (ABC) Transporters in Murine Melanoma Cells (B16-F1)

BDE-209 and TCDD Modulate the Expression and Activity of ATP-Binding Cassette (ABC) Transporters in Murine Melanoma Cells (B16-F1)

Review Data

Q: Is the topic relevant to the journal area of interest? Is it contemporary and interesting for


A: Good


Abstract & Keywords

Q: Are all required components included in the abstract? Are the keywords appropriately chosen?

A: Good



Q: Is the goal explicitly stated in the Introduction? Is its formulation clear and unambiguous?

A: Good



Q: Is the paper's structure coherent? Is it in coherence with the goal of the paper?

A: Good


Tools and Methods

Q: Are methods the author uses adequate and well used?

A: Good


Discussion & Conclusion

Q: Is it related to the results presented before? Do you consider them as coherent?

A: Good


Comments: The Discussion highlights the study on the effects of the exposure of BDE-209 and TCDD in the immortalized murine tumor cells (B16-F1) to evaluate the modulation of gene expression causing cell malignancy. It further discusses the upregulation and overexpression of the genes leading to cell malignancy, providing relevant literature. Lastly, the Discussion states that attention must be given to the ABC transporters responsible for the efflux of chemotherapeutic drugs for tumor cells in cancer susceptibility.



Q: Does the author utilize relevant literature?

A: Good


Author's knowledge

Q: What is the level of the author’s knowledge? Does the author utilize all recent contributions relevant to the topic?

A: Good



Q: Is the length of the paper adequate to the significance of the topic? Do you suggest shortening the paper without losing its value?

A: Good


Figures & Tables

Q: Does the author use them suitably? Are legend and notations clear?

A: Good.


Writing style

Q: Is it clear and understandable?

A: Good

Comments: There are few issues found in the manuscript which are as follows:

·       In Abstract, last sentence, “This investigation revealed … may intervene and modify \cells’ … resistance to chemotherapy” should be written as “This investigation revealed … might intervene and modify cells’ … resistance to chemotherapy”.

·       In Results, 3rd paragraph, 4th sentence, “Erro! Fonte de referência não encontrada.A” does not match with the intended meaning of the sentence. Hence the sentence is removed.

·       In Discussion, 3rd paragraph, 3rd sentence, “The relevance of regulation … related with the involvement of ABC transporters…” must be “The relevance of regulation … related to the involvement of ABC transporters …”.


Further comments on the paper

Comments: The manuscript describes the modulating levels of BDE-209 and TCDD in gene expression and activity of ATP-binding cassette (ABC) transporters in murine melanoma (B16-F1) cells. The study also illustrates the upregulation of the specific genes and change in the protein activity with exposure to BDE-209 and TCDD. The manuscript further provides the overall design of the study conducted, highlighting its statistical analysis.


Q: Would you recommend this manuscript for further publication?

A: Yes - Suitable to be published

If you have any questions and clarifications you can write to the journal.

Science Repository Team


Author Info

Corresponding Author
Ciro A. Oliveira Ribeiro
Cellular Toxicology Laboratory, Department of Cellular Biology, Biological Sciences Sector, Polytechnic Center/Federal University of Paraná, Curitiba, Paraná, Brazil

Article Info

Article Type
Research Article
Publication history
Received: Mon 09, Aug 2021
Accepted: Mon 23, Aug 2021
Published: Thu 02, Sep 2021
© 2023 Ciro A. Oliveira Ribeiro. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.IJCST.2021.02.01