The Pivotal Role of PAO in CDK Inhibitors (Roscovitine and Purvalanol)- Triggered Apoptosis in PUMA Null HCT116 Colon Cancer Cells

Review Data

Q: Is the topic relevant to the journal area of interest? Is it contemporary and interesting for

researchers?

A: Good

Abstract & Keywords

Q: Are all required components included in the abstract? Are the keywords appropriately chosen?

A: Good

Goal

Q: Is the goal explicitly stated in the Introduction? Is its formulation clear and unambiguous?

A: Good

Structure

Q: Is the paper's structure coherent? Is it in coherence with the goal of the paper?

A: Good

Tools and Methods

Q: Are methods the author uses adequate and well used?

A: Good

Discussion & Conclusion

Q: Is it related to the results presented before? Do you consider them as coherent?

A: Good

Comments: The Discussion briefly explains the importance of cyclins and cyclin-dependent kinases (CDKs) in the cell cycle, further discussing the significance of targeting the regulatory proteins mentioning relevant literature. Moving forward, it also highlights an overview of the role of polyamine oxidase (PAO) in CDKi-induced apoptosis. The Conclusion states that the alteration in PA catabolism is critical for CDKi-induced cell death.

Literature

Q: Does the author utilize relevant literature?

A: Good

Author's knowledge

Q: What is the level of author’s knowledge? Does the author utilize all recent contributions relevant to the topic?

A: Good

Length

Q: Is the length of the paper adequate to the significance of the topic? Do you suggest shortening the paper without losing its value?

A: Good

Figures & Tables

Q: Does the author use them suitably? Are legend and notations clear?

A: Good

Writing style

Q: Is it clear and understandable?

A: Good

Comments: There are few issues found in the manuscript which are as follows:

·       In Results under Abstract, 3^rd sentence, “Whereas drug-induced apoptosis was occurred caspase-independently in Puma-/- colon cancer cells” is reframed as “Whereas drug-induced apoptosis occurred caspase-independent in Puma-/- colon cancer cells”.

·       Under heading Introduction, 7^th sentence, “The polycationic amine derivatives, spermine, spermidine and putrescine, which referred as natural polyamines (PAs)..” must be “The polycationic amine derivatives, spermine, spermidine and putrescine, which are referred as natural polyamines (PAs)..”

·       Under Materials and Methods, in subheading Drugs and cell lines, 5^th sentence, “1% nonessential amino acids… in 5% CO₂ present  37°C incubator” must be written as “1% nonessential amino acids… in 5% CO₂ present in 37^°C incubator”.

·       In the subheading Determination of Cell Viability by MTT assay, 1^st word of last sentence must be “Absorbance” instead of “Absorbans”.

Further comments on the paper

Comments: The manuscript illustrates the pivotal role of polyamine oxidase (PAO) in CDK inhibitors-triggered apoptosis in PUMA knock-out HCT116 colon cancer cells. It describes the methods following the research. The results of the experiment are clearly stated in terms of the effect of polyamine expression in Cyclin-Dependent Kinase inhibitors (CDKi)-induced cell death in HCT116 colorectal cancer cells.

Q: Would you recommend this manuscript for further publication?

A: Yes - Suitable to be published

If you have any questions and clarifications you can write to the journal.

Thanks,
Science Repository Team