The Pivotal Role of PAO in CDK Inhibitors (Roscovitine and Purvalanol)- Triggered Apoptosis in PUMA Null HCT116 Colon Cancer Cells

The Pivotal Role of PAO in CDK Inhibitors (Roscovitine and Purvalanol)- Triggered Apoptosis in PUMA Null HCT116 Colon Cancer Cells

Review Data

Q: Is the topic relevant to the journal area of interest? Is it contemporary and interesting for


A: Good


Abstract & Keywords

Q: Are all required components included in the abstract? Are the keywords appropriately chosen?

A: Good



Q: Is the goal explicitly stated in the Introduction? Is its formulation clear and unambiguous?

A: Good



Q: Is the paper's structure coherent? Is it in coherence with the goal of the paper?

A: Good


Tools and Methods

Q: Are methods the author uses adequate and well used?

A: Good


Discussion & Conclusion

Q: Is it related to the results presented before? Do you consider them as coherent?

A: Good


Comments: The Discussion briefly explains the importance of cyclins and cyclin-dependent kinases (CDKs) in the cell cycle, further discussing the significance of targeting the regulatory proteins mentioning relevant literature. Moving forward, it also highlights an overview of the role of polyamine oxidase (PAO) in CDKi-induced apoptosis. The Conclusion states that the alteration in PA catabolism is critical for CDKi-induced cell death.



Q: Does the author utilize relevant literature?

A: Good


Author's knowledge

Q: What is the level of author’s knowledge? Does the author utilize all recent contributions relevant to the topic?

A: Good



Q: Is the length of the paper adequate to the significance of the topic? Do you suggest shortening the paper without losing its value?

A: Good


Figures & Tables

Q: Does the author use them suitably? Are legend and notations clear?

A: Good


Writing style

Q: Is it clear and understandable?

A: Good


Comments: There are few issues found in the manuscript which are as follows:

·       In Results under Abstract, 3rd sentence, “Whereas drug-induced apoptosis was occurred caspase-independently in Puma-/- colon cancer cells” is reframed as “Whereas drug-induced apoptosis occurred caspase-independent in Puma-/- colon cancer cells”.

·       Under heading Introduction, 7th sentence, “The polycationic amine derivatives, spermine, spermidine and putrescine, which referred as natural polyamines (PAs)..” must be “The polycationic amine derivatives, spermine, spermidine and putrescine, which are referred as natural polyamines (PAs)..”

·       Under Materials and Methods, in subheading Drugs and cell lines, 5th sentence, “1% nonessential amino acids… in 5% CO2 present  37°C incubator” must be written as “1% nonessential amino acids… in 5% CO2 present in 37°C incubator”.

·       In the subheading Determination of Cell Viability by MTT assay, 1st word of last sentence must be “Absorbance” instead of “Absorbans”.


Further comments on the paper

Comments: The manuscript illustrates the pivotal role of polyamine oxidase (PAO) in CDK inhibitors-triggered apoptosis in PUMA knock-out HCT116 colon cancer cells. It describes the methods following the research. The results of the experiment are clearly stated in terms of the effect of polyamine expression in Cyclin-Dependent Kinase inhibitors (CDKi)-induced cell death in HCT116 colorectal cancer cells.


Q: Would you recommend this manuscript for further publication?

A: Yes - Suitable to be published

If you have any questions and clarifications you can write to the journal.

Science Repository Team


Author Info

Corresponding Author
Ajda Coker-Gurkan
Department of Molecular Biology and Genetics, Faculty of Engineering and Life Sciences, Topkapi Campus, Biruni University, Topkapi, Istanbul, Turkey

Article Info

Article Type
Research Article
Publication history
Received: Wed 07, Jul 2021
Accepted: Wed 21, Jul 2021
Published: Wed 04, Aug 2021
© 2023 Ajda Coker-Gurkan. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.IJCST.2021.01.03