Plasma Treatment Effects on Oral Candida albicans Biofilms

Plasma Treatment Effects on Oral Candida albicans Biofilms

Review Data

Q: Is the topic relevant to the journal area of interest? Is it contemporary and interesting for researchers?

A: Very good


Abstract & Keywords

Q: Are all required components included in the abstract? Are the keywords appropriately chosen?

A: Very good



Q: Is the goal explicitly stated in the Introduction? Is its formulation clear and unambiguous?

A: Very good



Q: Is the paper's structure coherent? Is it in coherence with the goal of the paper?

A: Good


Tools and Methods

Q: Are methods the author uses adequate and well used?

A: Very good


Discussion & Conclusion

Q: Is it related to the results presented before? Do you consider them as coherent?

A: Excellent


Comments: The Discussion section, with the help of adequate relevant literature, determines the efficacy of plasma treatment effects on Candida albicans biofilm decontamination in vitro. Based on the findings and relevant literature, it demonstrates that low-temperature atmospheric plasma treatment can significantly disinfect C. albicans biofilm. It also highlights the importance of the combination of plasma and chlorhexidine digluconate (CHX) treatment which has a potential to increase the antifungal efficiency against C. albicans biofilms. In conclusion, the low-temperature plasma treatment is effective in deactivating Candida albicans biofilms and thus may serve as a promising alternative to disinfect oral fungal biofilms. The limitation of this study is also properly addressed.



Q: Does the author utilize relevant literature?

A: Very good


Author's knowledge

Q: What is the level of the author’s knowledge? Does the author utilize all recent contributions relevant to the topic?

A: Very good



Q: Is the length of the paper adequate to the significance of the topic? Do you suggest shortening the paper without losing its value?

A: Good


Figures & Tables

Q: Does the author use them suitably? Are legend and notations clear?

A: Excellent


Writing style

Q: Is it clear and understandable?

A: Good



·       Words like “fourth”, “solubilization”, “Dimensional, “rupture”, “red”, “fungal”, “treatment” are misspelled in the text section of the manuscript.

·       In the 6th sentence of the “Confocal laser scanning microscope (CLSM) analysis” under “Materials and methods” section, the word “emerged” should be replaced with “immersed”.


Further comments on the paper

Comments: This study investigates the effect of antimicrobial efficacy of low-temperature atmospheric plasma treatment in the disinfection of Candida albicans biofilm in vitro. Candida albicans is an asexual diploid fungus responsible for causing oral candidiasis. This study evaluates the plasma treatment effects on Candida albicans biofilms and the efficacy is determined by 3-(4,5-dimethylazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and confocal laser scanning microscope (CLSM) analysis. The findings indicate that plasma treatment causes a significant reduction of survival rate of C. albicans and a higher plasma power provides better disinfection efficiency. It also assesses the synergistic effects of plasma treatment with chlorhexidine digluconate (CHX) against C. albicans biofilms that establishes that the combination of plasma treatment and CHX treatment offers better deactivation effects than single plasma treatment and provides valuable insights into the effectiveness of gas plasma treatment which can be a promising alternative to disinfect oral fungal biofilms. However, the complicated setup of the plasma treatment is the limitation of this study.


Q: Would you recommend this manuscript for further publication?

A: Yes - Suitable to be published

If you have any questions and clarifications you can write to the journal.

Science Repository Team


Author Info

Corresponding Author
Qingsong Yu
Department of Mechanical and Aerospace Engineering, University of Missouri, Columbia, Missouri, USA

Article Info

Article Type
Research Article
Publication history
Received: Wed 28, Apr 2021
Accepted: Fri 14, May 2021
Published: Tue 01, Jun 2021
© 2021 Qingsong Yu. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository. All rights reserved.
DOI: 10.31487/j.DOBCR.2021.02.05