Combined Bacterial Antigen Lipopolysaccharide and Lipoteichoic Acid Increase Cal 27 Oral Cancer Cell Proliferation

Review Data

Q: Is the topic relevant to the journal area of interest? Is it contemporary and interesting for researchers?

A: Very good

Abstract & Keywords

Q: Are all required components included in the abstract? Are the keywords appropriately chosen?

A: Very good

Goal

Q: Is the goal explicitly stated in the Introduction? Is its formulation clear and unambiguous?

A: Very good

Structure

Q: Is the paper's structure coherent? Is it in coherence with the goal of the paper?

A: Very good

Tools and Methods

Q: Are methods the author uses adequate and well used?

A: Very good

Discussion & Conclusion

Q: Is it related to the results presented before? Do you consider them as coherent?

A: Excellent

Comments: The Discussion section summarizes from the results that the different effects of combined bacterial antigen lipopolysaccharide (LPS) and lipoteichoic acid (LTA) increase the progression of oral cancer cell proliferation on Cal 27 oral cell line. This study provides insights for all the probable pathways that get affected due to bacterial antigen stimulation effects which result in downregulation of tumor-suppressor proteins and upregulation of proliferation-related proteins, leading to oral cancer progression.

Literature

Q: Does the author utilize relevant literature?

A: Very good

Author's knowledge

Q: What is the level of the author’s knowledge? Does the author utilize all recent contributions relevant to the topic?

A: Very good

Length

Q: Is the length of the paper adequate to the significance of the topic? Do you suggest shortening the paper without losing its value?

A: Good

Figures & Tables

Q: Does the author use them suitably? Are legend and notations clear?

A: Excellent

Writing style

Q: Is it clear and understandable?

A: Good

Comments:

·       Words like “nucleatum” and “anaerobic” are misspelled in the text section of the manuscript.

Further comments on the paper

Comments: This study compares the differential effects of combined bacterial antigen lipopolysaccharide (LPS) and lipoteichoic acid (LTA) with the stimulation by LPS alone on oral cancer cell lines in order to elucidate their different actions on oral cancer cell proliferation. It investigates the possible pathways that may be affected by these bacterial antigens. The findings show that Cal 27 after LPS+LTA stimulation produced the highest proliferation among all the oral cell lines. This study calls for further research on exploring bacterial components other than bacterial cell wall antigens, involved in the progression of oral squamous cell carcinoma on different oral cell lines.

Q: Would you recommend this manuscript for further publication?

A: Yes - Suitable to be published

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Science Repository Team