Bfp Adhesions and EspA Filament by Enteropathogenic E.Coli in Relation to Pathogenesis of Infantile Diarrhea in Children Under 5 Years in Mansoura University Children Hospital
Corresponding AuthorGhada Barakat
Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt
A B S T R A C T
Background: Our study was applied aiming to detect the biofilm formation by the Enteropathogenic E. coli causing diarrhea and to investigate the relation between the Bfp adhesions and EspA filament containing strains with the severity of diarrhea in children under 5 years in Mansoura University Children Hospital, Egypt. Methods: It was carried out by identification of EPEC, Serological agglutination test, Biofilm Formation under static condition at 3,6,9,24 hours. Results: Out of 103 children with diarrhea, 50 E. coli were isolated, 16 (32%) were EPEC and 34 (68%) were non-EPEC. The CFU mean± SD of biofilm of 16 EPEC isolates at 4 time points 3, 6, 9, 24h are 108- 1013, 69.5910±24.9310, 106-109, 20.2948±3.96778, 107-1011, 13.92×1010±3.376×1010, 109-1015, 6.402×1013± 2.496×1013. The relation of the biofilm formation and the severity of diarrhea in 16 EPEC isolates including 5 typical EPEC isolates and11 atypical EPEC reveals significant relation between the duration of the diarrhea and biofilm formation P=0.001**. Conclusion: EPEC is capable of forming biofilms on plastic under static and flow through-continuous conditions which correlate to the severity of the diarrhea in children under 5 years.
Article TypeResearch Article
Publication historyReceived: Thu 26, Mar 2020
Accepted: Wed 08, Apr 2020
Published: Mon 13, Apr 2020
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