Evaluation of the Number, Biophysical and Multipotent Characteristics of Adipose Derived Stem Cells Harvested by SEFFI Procedure and Interaction with Different Type of Hyaluronic Acids

Evaluation of the Number, Biophysical and Multipotent Characteristics of Adipose Derived Stem Cells Harvested by SEFFI Procedure and Interaction with Different Type of Hyaluronic Acids

Review Data

Q: Is the topic relevant to the journal area of interest? Is it contemporary and interesting for


A: Good


Abstract & Keywords

Q: Are all required components included in the abstract? Are the keywords appropriately chosen?

A: Good



Q: Is the goal explicitly stated in the Introduction? Is its formulation clear and unambiguous?

A: Good



Q: Is the paper's structure coherent? Is it in coherence with the goal of the paper?

A: Good


Tools and Methods

Q: Are methods the author uses adequate and well used?

A: Good


Discussion & Conclusion

Q: Is it related to the results presented before? Do you consider them as coherent?

A: Good


Comments: The results are merged with the Discussion providing an overview of the feasibility study, cellularity, proliferation, senescent phenotype, mesenchymal stem cells (MSCs) phenotype and adipogenic, osteogenic and chondrogenic differentiation mentioning relevant literature. The study discusses the possibility of deriving vital stromal vascular fraction (SVF) cells and adipocytes from lipoaspirate samples harvested with the SEFFI procedure. Lastly, the Conclusion states that the development of a new fat tissue product, SEFFI + HA, can be available for the ready-to-use treatment of human skin aging and open the novel strategy in personalized regenerative medicine.



Q: Does the author utilize relevant literature?

A: Good


Author's knowledge

Q: What is the level of author’s knowledge? Does the author utilize all recent contributions relevant to the topic?

A: Good



Q: Is the length of the paper adequate to the significance of the topic? Do you suggest shortening the paper without losing its value?

A: Good


Figures & Tables

Q: Does the author use them suitably? Are legend and notations clear?

A: Good


Writing style

Q: Is it clear and understandable?

A: Good


Comments: There are some minor errors found in the manuscript, which are as follows:

·       Under Introduction, 1st paragraph, 3rd sentence, “ADSCs have shown to possess the in vitro…” should be written as “ADSCs have been shown to possess the in vitro…”.

·       Students’ t-test must be written as “Student’s t-test all throughout the manuscript.


Further comments on the paper

Comments: The manuscript evaluates the number, biophysical and multipotent characteristics of adipose-derived stem cells harvested by the SEFFI procedure and highlights the interaction with different hyaluronic acids. The study further combines the grafting of micro fragmented fatty tissue with the hyaluronic acid filler procedure, using three different types of HA. The study’s outcome illustrates that the combination of emulsified harvested tissue with HA can be utilized to counteract the loss of volume and skin aging of the human face and body. The research establishes the approach to regenerative aesthetic treatment as a promising procedure for facial antiaging therapy.


Q: Would you recommend this manuscript for further publication?

A: Yes - Suitable to be published

If you have any questions and clarifications you can write to the journal.

Science Repository Team


Author Info

Corresponding Author
Alessandro Gennai
Plastic Surgeon, Private Practice, Studio Gennai, Bologna, Italy

Article Info

Article Type
Research Article
Publication history
Received: Fri 10, Sep 2021
Accepted: Thu 07, Oct 2021
Published: Thu 21, Oct 2021
© 2023 Alessandro Gennai. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.RGM.2021.02.02