Effects of the Zinc Finger Protein 485 (ZNF485) on the Proliferation, Metastasis and Invasion of Bladder Cancer Cells

A B S T R A C T

Objectives: Bladder cancer is the second most common urological cancer worldwide with low early diagnosis and high mortality. The limited progress on the diagnostics and treatment largely impedes the survival of bladder cancer patients.
Methods: Potential therapeutic biomarkers are urgently needed for future clinic treatment. We performed the RNA-seq assays and identified a new gene zinc finger protein 485, termed ZNF485, which is highly expressed in the tissues of bladder cancer patients.
Results: We found that inhibition of ZNF485 in bladder cancer cell line T24 and 5637 can obviously inhibit the proliferation and promotes the apoptosis of cancer cells. Furthermore, the wound healing and invasion assays showed that down-regulation of ZNF485 significantly decreased the mobility and invasion of T24 and 5637 cells. In addition, ZNF485-siRNA transfected obviously inhibited tumor growth in nude mice.
Conclusion: Taken together, the results provide evidence that ZNF485 is involved in the tumorigenesis of bladder cancer, which might be a potential therapeutic biomarker for the treatment of this disease.

Keywords

Bladder cancer, ZNF485, proliferation, metastasis, invasion



Get access to the full version of this article.

Article Info

Article Type
Research Article
Publication history
Received: Tue 13, Jul 2021
Accepted: Thu 29, Jul 2021
Published: Fri 20, Aug 2021
Copyright
© 2023 Dabing Huang. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.COR.2021.08.03

Author Info

Yiao Tan Fangfang Zhao Chunbao Zang Shuhan Liu Tao Huang Lingsuo Kong Fangfang Ge Dabing Huang Youguang Pu

Corresponding Author
Dabing Huang
Department of Oncology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, P.R. China

Figures & Tables



Get access to the full version of this article.

References

1.     Share JB (1976) Review of drug treatment for Down's syndrome persons. Am J Ment Defic 80: 388-393. [Crossref]

2.     Ehrhart IC, Parker PE, Weidner WJ, Dabney JM, Scott JB et al. (1975) Coronary vascular and myocardial responses to carotid body stimulation in the dog. Am J Physiol 229: 754-760. [Crossref]

3.     Stamm O, Latscha U, Janecek P, Campana A (1976) Development of a special electrode for continuous subcutaneous pH measurement in the infant scalp. Am J Obstet Gynecol 124: 193-195. [Crossref]

4.     Bland RD, Clarke TL, Harden LB (1976) Rapid infusion of sodium bicarbonate and albumin into high-risk premature infants soon after birth: a controlled, prospective trial. Am J Obstet Gynecol 124: 263-267. [Crossref]

5.     Lv L, Deng H, Li Y, Zhang C, Liu X et al. (2014) The DNA methylation-regulated miR-193a-3p dictates the multi-chemoresistance of bladder cancer via repression of SRSF2/PLAU/HIC2 expression. Cell Death Dis 5: e1402. [Crossref]

6.     Poole Wilson PA, Langer GA (1975) Effect of pH on ionic exchange and function in rat and rabbit myocardium. Am J Physiol 229: 570-581. [Crossref]

7.     Ma K, He Y, Zhang H, Fei Q, Niu D et al. (2012) DNA methylation-regulated miR-193a-3p dictates resistance of hepatocellular carcinoma to 5-fluorouracil via repression of SRSF2 expression. J Biol Chem 287: 5639-5649. [Crossref]

8.     Moroi K, Sato T (1975) Comparison between procaine and isocarboxazid metabolism in vitro by a liver microsomal amidase-esterase. Biochem Pharmacol 24: 1517-1521. [Crossref]

9.     Lv L, Li Y, Deng H, Zhang C, Pu Y et al. (2015) MiR-193a-3p promotes the multi-chemoresistance of bladder cancer by targeting the HOXC9 gene. Cancer Lett 357: 105-113. [Crossref]

10.  Deng H, Lv L, Li Y, Zhang C, Meng F et al. (2015) The miR-193a-3p regulated PSEN1 gene suppresses the multi-chemoresistance of bladder cancer. Biochim Biophys Acta 1852: 520-528. [Crossref]

11.  Tang Z (2018) Effects of human enhancer of filamentation 1 (HEF1) gene on the proliferation, invasion and metastasis of bladder cancer cells. J BUON 23: 782-786. [Crossref]

12.  Garcia Perdomo HA, Usubillaga Velasquez JP, Zapata Copete JA, Reis LO (2019) Mutations in CDKN2A and the FGFR3 genes on bladder cancer diagnosis: a systematic review and meta-analysis. World J Urol 37: 2001-2007. [Crossref]

13.  Pu Y, Zhao F, Wang H, Cai W, Gao J et al. (2016) MiR-34a-5p promotes the multi-drug resistance of osteosarcoma by targeting the CD117 gene. Oncotarget 7: 28420-34. [Crossref]

14.  Pu Y, Zhao F, Li Y, Cui M, Wang H et al. (2017) The miR-34a-5p promotes the multi-chemoresistance of osteosarcoma via repression of the AGTR1 gene. BMC Cancer 17: 45. [Crossref]

15. Pu Y, Zhao F, Wang H, Cai S (2017) MiR-34a-5p promotes multi-chemoresistance of osteosarcoma through down-regulation of the DLL1 gene. Sci Rep 7: 44218. [Crossref]