Overexpression of BMP-2 in mesenchymal stem cells with amplifying virus-like particles
Corresponding AuthorSteve Stice
Regenerative Bioscience Center, University of Georgia, 425 River Road, Athens GA 30602, USA
A B S T R A C T
There are numerous strategies under development to supplement or replace bone grafts. Among these synthetic options, growth factors such as bone morphogenetic protein (BMP-2) are often used. However, current burst delivery strategies have been associated with a number of complications. Transplanting cells engineered to over-express osteoinductive proteins over a longer period of time is a possible solution to this problem. In this study, we demonstrated the use of a parainfluenza virus 5 (PIV-5) vector (amplifying virus-like particles, AVLP) to induce expression of exogenous proteins in mesenchymal stem cells (MSC). We obtained extended expression of enhanced green fluorescent protein eGFP in transduced MSCs, as well as selection and maintenance of eGFP+ cells. We also attained expression of BMP-2 on a shorter timescale using this method. We conclude that AVLP is a promising tool for genetic engineering, both in regenerative medicine and other applications.
Article TypeResearch Article
Publication historyReceived: Wed 18, Jul 2018
Accepted: Mon 06, Aug 2018
Published: Tue 28, Aug 2018
Copyright© 2019 Steve Stice. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository. All rights reserved.