Exploration of Endophytes from Alfalfa (Medicago sativa L.) as Biocontrol Agents

Author Info

Beilei Wu Mei Li Xiaoli Chen Xiliang Jiang Xin Wang

Corresponding Author

Beilei Wu
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China

A B S T R A C T

Endophytes are increasingly investigated as biocontrol agents for agricultural production. The identification of new endophytes with high effectiveness against plant disease is very important. A total of 362 strains of endophytes, including fungi, bacteria, and actinomycete, were isolated from alfalfa (Medicago sativa L.) collected in Hebei, Inner Mongolia and Ningxia provinces of China. The three strains of endophytic bacteria (NA NX51R-5, NA NX90R-8, and NA NM1S-1) with strong biocontrol capability with >50% effectiveness were screened against the common alfalfa root rot pathogen Fusarium oxysporum F. sp. medicaginis in alfalfa seedling germination experiments on MS medium and pot experiments. Using phylogenetic analysis, the isolates of NA NM1S-1 and NA NX51R-5 were identified as Bacillus spp. by 16S rDNA, while NA NX90R-8 was found to be Pseudomonas sp.

Article Info

Article Type

Research Article

Publication history

Received: Fri 28, Feb 2020
Accepted: Thu 12, Mar 2020
Published: Mon 30, Mar 2020

Copyright

© 2023 Beilei Wu. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.GG.2020.01.01