No Association was Detected between Polymorphism of COL1A2 Genes and Occurrence of Dental Fluorosis Among the Subjects Living in a Fluorosis-Endemic Area of India

Author Info

Chitta Chowdhury Shahnawaz Khijmatgar Mohammed S Mustak Avidyuti Chowdhury Divya Kumari P Edward Lynch Martin Grootveld

Corresponding Author

Mohammed S Mustak
Department of Applied Zoology, Mangalore University, Mangalagangothri, Mangalore, Karnataka, India

A B S T R A C T

Objective: To determine any genetic association of COL1A2 polymorphism and the occurrence of dental fluorosis within an Indian human dental fluorosis population. Material and Methods: Fifty-six (56) subjects from two groups i.e. cases with dental fluorosis from the Pavagada population (n=29) and a control group (n=27) without fluorosis, were explored. The ages ranged between 15 and 76 years (mean 50.8 years) were included, and the male to female ratio was 70:30. The severity of dental fluorosis was graded using WHO’s Thylstrup-Fejerskov index (TF), and the concentration of fluoride was determined by a fluoride ion selective electrode (ISE). Genomic DNA was extracted using the standard phenol-chloroform method. The rs412777 and rs414408 polymorphism in COL1A2 were genotyped using the Sanger sequence method. Results: Genotype distributions for rs412777 within each group were: AA 41%, AC 51%, and CC 7% for dental fluorosis participants, and AA 56%, AC 46%, and CC 0% for the control participants. Conclusions: The rs412777 and rs414408 polymorphisms in the COL1A2gene showed no significant association between COL1A2 and the occurrence of dental fluorosis amongst this Indian population.

Article Info

Article Type

Research Article

Publication history

Received: Wed 01, Apr 2020
Accepted: Mon 20, Apr 2020
Published: Mon 26, Apr 2021

Copyright

© 2023 Mohammed S Mustak. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.DOBCR.2020.02.04