Enamel Matrix Derivative and TGF-Beta 1 Target Genes in Human Tongue Carcinoma Cells

Enamel Matrix Derivative and TGF-Beta 1 Target Genes in Human Tongue Carcinoma Cells

Review Data

Q: Is the topic relevant to the journal area of interest? Is it contemporary and interesting for researchers?

A: Excellent


Abstract & Keywords

Q: Are all required components included in the abstract? Are the keywords appropriately chosen?

A: Very good



Q: Is the goal explicitly stated in the Introduction? Is its formulation clear and unambiguous?

A: Very good



Q: Is the paper's structure coherent? Is it in coherence with the goal of the paper?

A: Very good


Tools and Methods

Q: Are methods the author uses adequate and well used?

A: Very good


Discussion & Conclusion

Q: Is it related to the results presented before? Do you consider them as coherent?

A: Very good



The study is well designed and executed. Methods and Results are adequately described. The Discussion offers a thorough and detailed analysis of the findings of the present study taking all the relevant literature into account. 



Q: Does the author utilize relevant literature?

A: Very good


Author's knowledge

Q: What is the level of the author’s knowledge? Does the author utilize all recent contributions relevant to the topic?

A: Very good



Q: Is the length of the paper adequate to the significance of the topic? Do you suggest shortening the paper without losing its value?

A: Very good


Figures & Tables

Q: Does the author use them suitably? Are legend and notations clear?

A: Very good

Comments: The legends for supplementary tables S1 and S2 were missing and were therefore inserted as S1: Genes statistically significantly (P<0.05) up-regulated and down-regulated after 6 h of TGF-beta 1 treatment; S2: Genes statistically significantly (P<0.05) up-regulated and down-regulated after 24 h of TGF-beta 1 treatment.



Writing style

Q: Is it clear and understandable?

A: Very good

Comments: A few minor issues were noted –

·       In the 4th sentence of the Abstract, “200 microg/ml” must be replaced with “200 µg/ml” to maintain consistency in the manuscript.

·       In the 2nd paragraph of the Introduction, in the 2nd sentence, “showed” must be replaced with “shown”, and in the last sentence, “suggest” must be replaced with “suggests”.

·       In the 1st sentence of the 3rd paragraph of the Introduction, “acts also” must be replaced with “also acts”.

·       “,” before “that” must be removed, e.g., after “concluded” in the last sentence of the 3rd paragraph of the Introduction, after “shown” in the 1st sentence of the 4th paragraph of the Introduction, and so on.

·       In Microarray sample preparation and hybridization under Materials and Methods, in the 5th sentence of “Qiagen” is misspelled.

·       Commas and articles (a, an, or the) were missing in several places.


Further comments on the paper

Comments: This study was the first microarray to compare the effects of enamel matrix derivative (EMD) and TGF-beta 1 on highly malignant invasive oral carcinoma cells (HSC-3). TGF-beta 1 was found to be more effective and showed a different gene expression pattern compared with EMD for 6h and 24h incubations. Both EMD and TGF-beta 1 statistically significantly induced the expression of MMP-9, but their effects on MMP-1 and -10 varied.


Q: Would you recommend this manuscript for further publication?

A: Yes - Suitable to be published

If you have any questions and clarifications you can write to the journal.

Science Repository Team


Author Info

Corresponding Author
Matti Mauramo
Department of Pathology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland

Article Info

Article Type
Research Article
Publication history
Received: Thu 10, Dec 2020
Accepted: Sat 26, Dec 2020
Published: Thu 31, Dec 2020
© 2021 Matti Mauramo. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository. All rights reserved.
DOI: 10.31487/j.DOBCR.2020.06.05