Chunpu  Li,Sicheng  Wang,Lili  Zhao,Dongmei Guo,Jilong Yang ,
Overexpression of SP6 Correlates with Osteosarcoma Metastasis and Poor Prognosis
Journal of Surgical Oncology
2020
2674-3000
http://dx.doi.org/10.31487/j.JSO.2020.04.06
https://www.sciencerepository.org/overexpression-of-sp6-correlates-with-osteosarcoma-metastasis-and-poor-prognosis_JSO-2020-4-106 
Abstract: Background: SP6 (Specificity protein 6) has been explored as a prospective biomarker in several cancers.
In this research, the prognostic value of SP6 expression in osteosarcoma was predicted by bioinformatics
analysis. Data were obtained from the Gene Expression Omnibus (GEO) database.
Methods: Gene expression data and clinical materials were downloaded from the GSE21257 dataset. The
mRNA expression of SP6 was compared between metastatic and non-metastatic tissues with the Wilcoxon
rank-sum test, and the relationship between SP6 and clinicopathological characters was analysed using
logistic regression. In addition, the correlation between SP6 and survival rate was assessed using KaplanMeier and Cox regression. Moreover, receiver operating characteristic (ROC) curve analysis was conducted
to determine the prognostic merit of SP6 for osteosarcoma. The biological functions of SP6 were annotated
and evaluated through gene set enrichment analysis (GSEA) and gene set variation analysis (GSVA).
Results: SP6 was significantly highly expressed in metastatic osteosarcoma tissues (p = 0.002). High SP6
expression showed a positive correlation with Huvos grade (OR = 6.60 for I vs. II, p = 0.028). The overall
survival (OS) of the patients with high SP6 expression was significantly poorer than the low SP6 expression
group (p = 0.027). The multivariate analysis revealed that SP6 expression (p = 0.002, HR = 15.40 (95% CI
[2.84–83.44])) was independently correlated with OS. GSEA and GSVA showed that "spliceosome" and
"base excision repair" were significantly upregulated in the high expression group of SP6.
Conclusion: SP6 may serve an independent prognostic biomarker in osteosarcoma.Keywords: Osteosarcoma, SP6, metastasis, prognosis, biomarker