Bekee C.L,Jesumirhewe Christiana, Antimicrobial Activity of Honey Samples from Ovia North East Local Government Areas in Edo State, Nigeria Journal of Food Nutrition and Metabolism 2019 2674-2411 http://dx.doi.org/10.31487/j.JFNM.2019.04.01 https://www.sciencerepository.org/antimicrobial-activity-of-honey-samples-from-ovia-north-east-local-government-areas-in-edo-state-nigeria_JFNM-2019-4-101 Abstract: Background: Honey is one of the oldest natural products in our environment used as natural alternatives for emerging and re-emerging infectious diseases. Objectives: This study was carried out to determine the antimicrobial activity of some honey samples collected from Ovia North-East local government area of Edo state Nigeria. Materials and Methods: Three honey samples were obtained in April 2017 from local commercial producers in Ovia North-East local government area of Edo state Nigeria. Bacteria strains of Staphylococcus aureus, Escherichia coli, Proteus spp, Klebsiella spp and Pseudomonas aeruginosa were obtained from the University of Benin Teaching Hospital Edo state Nigeria. The antimicrobial activity of the honey samples was performed using the Kirby-Bauer disc diffusion method. The antimicrobial activity of Ciprofloxacin was also evaluated as a positive control. Results: All the honey samples had antimicrobial activities against the pathogens tested. Most of the strains showed the highest inhibition zone and hence better antimicrobial activity when the sample from Ogbese was tested against the strains. An increase in the honey concentration increased the activity of the sample and hence the zone of inhibition against the pathogens tested. Among the Bacteria strains tested Staphylococcus aureus and Pseudomonas aeruginosa were the most sensitive against all the honey samples. Conclusion: Our study shows the antimicrobial activity of honey samples from Ovia North east local government area in Edo state Nigeria. As bacterial resistance to antibiotics is on the increase, it is urgently required to discover alternative therapeutic agents. Keywords: Antimicrobial activity, Honey, Bacteria strains, Therapeutic agents