Babu  J.P,Banzi  E.C.F,Correr-Sobrinho  L,Costa  A.R,Garcia-Godoy  F,Puppin-Rontani  R.M,Tipton  DA,
Long-term dental adhesive toxicity on human gingival fibroblasts and epithelial cells
Dental Oral Biology and Craniofacial Research
2019
2613-4950
http://dx.doi.org/10.31487/j.DOBCR.2019.03.01
https://www.sciencerepository.org/long-term-dental-adhesive-toxicity-on-human-gingival-fibroblasts-and-epithelial-cells_DOBCR-2019-3-101 
Abstract: Dentin adhesive system (DAS) components released can induce cytotoxic effects. We hypothesize that
media obtained from long-term stored DAS are cytotoxic, depending on the DBS type/composition and
modulation of cytotoxicity is time dependent. 0.2 mL DMEM media obtained from incubating DAS discs
were filter-sterilized, added to the cultured cells (human gingival fibroblast and epithelial cells) for 24 hours
and harvested up to 50 days. Cell toxicity was assessed using MTT cell viability assay and LIVE/DEAD
backlight staining. PB was cytotoxic to both cell types only from 1h to 3 days. SE was non-toxic up to 1h,
but it was up to 50th day, when the toxicity ceased. The SB was slightly more toxic than Scotch, but by day
45 no significant toxicity to the cells was observed. We concluded that all DAS tested were toxic for the
cells analyzed, and cytotoxicity was composition/time dependent.Keywords: Cytotoxicity, adhesive systems, human gingival epithelial cells, human gingival fibroblast cells, MTT assay, live/dead assay