Feixin Liang,Tao Yu,Xiangming  Qin,
Effect of reactive oxygen species on the ligand-independent activation of EGFR in tongue squamous cell carcinoma
Dental Oral Biology and Craniofacial Research
2018
2613-4950
http://dx.doi.org/10.31487/j.DOBCR.2018.02.005
https://www.sciencerepository.org/effect-of-reactive-oxygen-species-on-the-ligand-independent-activation-of-EGFR-in-tongue-squamous-cell-carcinoma_DOBCR-2-105 
Abstract: Objective: To explore the expression of 8-hydroxy-2′-deoxyguanosine (8-OHdG), p-Src, and phosphorepidermal growth factor receptor (p-EGFR) and the effect of reactive oxygen species on ligand-independent
activation of EGFR in tongue squamous cell carcinoma (TSCC) tissues.
Methods: The expressions of 8-OHdG, EGFR, and p-EGFR in carcinoma tissues and carcinoma-adjacent
tissues of 40 cases of TSCC were determined immunohistochemically. The correlations between 8-OHdG
expression and p-Src and p-EGFR expressions were analyzed.
Results: 8-OHdG, p-Src, and p-EGFR were highly expressed in TSCC tissues, with positivity rates of
82.5%, 70%, and 55%, respectively, whereas they were lowly expressed in carcinoma-adjacent tissues, with
positivity rates of 17.5%, 12.5%, and 7.5%, respectively. The expressions of 8-OHdG, p-Src, and p-EGFR
were significantly higher in carcinoma tissue than in carcinoma-adjacent tissue (P < 0.05), and statistically
significant correlations were observed between 8-OHdG expression and p-Src and p-EGFR expressions (P
< 0.05).
Conclusion: This study preliminarily determined the expression of the reactive oxygen species-Src-EGFR
signaling pathway in TSCC, providing clinical evidence for the use of cetuximab in combination with other
chemotherapy drugs in the treatment of oral cancer.Keywords: ROS p-Src, p-EGFR, TSCC, Cetuximab