Magdy  m. Mohamed,Nashwa EL-Khazragy,Odett  M. Zaky,
Upregulation of HOTAIR Predicts Poor Outcome in Acute Myeloid Leukemia
Clinical Oncology and Research
2019
2613-4942
http://dx.doi.org/10.31487/j.COR.2019.04.03
https://www.sciencerepository.org/upregulation-of-hotair-predicts-poor-outcome-in-acute-myeloid-leukemia_COR-2019-4-103 
Abstract: Background: Acute myeloid leukemia (AML) is a clonal hematopoietic malignancy, in spite of the marked
improvement in the treatment of AML; Molecular biomarkers open the door to improve disease outcome.
Accumulating evidence suggested that the long non-coding RNA “HOTAIR” has an oncogenic role in
hemopoietic malignancies. Recently, it has been evident that knockdown of HOTAIR inhibits cell
proliferation and induces apoptosis by modulating c-Kit expression via acting as competing for endogenous
RNAs (ceRNAs) to sponge miR-193a at the post-transcriptional level.
Objectives: we aimed to evaluate the diagnostic and prognostic value of HOTAIR in AML, to investigate
its association with and c-Kit and miR-193a.
Subjects &Methods: we examined the expression levels of HOTAIR, miR-193a, and c-Kit in 100 de-novo
AML patients using quantitative, the association of genes expressions with risk factors and patient’s
outcome were statistically analyzed.
Results: the expression of HOTAIR was significantly upregulated by four folds in AML compared to
healthy controls; higher expression levels were associated with high-risk factors, poorer overall survival
(OS) and shorter leukemia-free survival (LFS). In addition; a negative correlation was detected between
Lnc-HOTAIR and miR-193a, although significance didn’t reach.
Conclusion: The obtained results suggested that HOTAIR expression was upregulated in peripheral blood
samples of de-novo AML patients and was associated with leukemic burden and disease outcome.
Therefore, it may represent an effective diagnostic and poor prognostic tool for AML.Keywords: HOTAIR, Leukemia, Myeloid