TY - JOUR
AR - RDI-2019-2-103
TI - Radiosensitizing performance of uncoated and citrate-coated SPIONs in cancerous and non-cancerous cells
AU -  W. , Neuhuber
AU - Carola , Kryschi
AU - Damjana , Drobne
AU - Luitpold V.,  R. Distel
AU - Stefanie , Klein
AU - Veno , Kononenko
JO - Radiology and Medical Diagnostic Imaging
PY - 2019
DA - Mon 22, Jul 2019
SN - 2613-7836
DO - http://dx.doi.org/10.31487/j.RDI.2019.02.03
UR - https://www.sciencerepository.org/radiosensitizing-performance-of-uncoated-and-citrate-coated-spions-in-cancerous-and-non-cancerous-cells_RDI-2019-2-103 
KW - Superparamagnetic iron oxide nanoparticles, radiation therapy, cellular uptake, enzyme activity, intracellular hydrogen peroxide
AB - Superparamagnetic iron oxide nanoparticles were shown to exhibit a high performance as X-ray dosage
enhancer in tumor cells. The radio-enhancing potential of uncoated and citrate-coated SPIONs was
comprehensively studied for tumor and healthy cells. Pristine and citrate-coated SPIONs drastically differ
in their water dispersibility and adsorption affinity for proteins. The activities of antioxidant enzymes in the
healthy cells were shown to be significantly distinct from those in the tumor cells as containing a much
higher H2O2 concentration. Pristine SPIONs catalyzed the Fenton reaction of hydrogen peroxide to the
highly reactive hydroxyl radical in all cell types. In contrast, intracellular citrate-stabilized SPIONs were
shown t
o be non-toxic and to do not affect the formation of reactive oxygen species. X-ray irradiation of citratestabilized SPIONs, when internalized by tumor cells, significantly boost the formation of hydroxyl radicals,
whereas the healthy cells preserved their initial levels of reactive oxygen species.