TY - JOUR
AR - DOBCR-2019-1-101
TI - Review of Oral Probiotics and the Methods Useful in Study of Dental Biofilms and for Selection of Potential Beneficial Bacteria and Their Products for Development of Oral Probiotic
AU - Englerová, Karolína
AU - Fedorov? , Monika
AU - Gabriela, ?trkolcov?
AU - Jana , Ka??rov?
AU - Lauko , Stanislav
AU - Madar , Marian
AU - Nemcov? , Radom?ra
AU - Sona , Gancarcikova
AU - Sta?kov? , Andrea
AU - Stykov? , Eva
JO - Dental Oral Biology and Craniofacial Research
PY - 2019
DA - Thu 31, Jan 2019
SN - 2613-4950
DO - http://dx.doi.org/10.31487/j.DOBCR.2019.01.001
UR - https://www.sciencerepository.org/oral-probiotics-and-the-methods-useful-in-study-of-dental-biofilms-and-for-selection-of-potential-beneficial-bacteria_SR-DOBCR-2019-1-101 
KW - Potential oral beneficial bacteria methods, streptococcus, lactobacillus
AB - Colonization of the oral cavity begins immediately after birth, however, only some micro-organisms are
capable of exerting their action in the oral environment. A wide range of micro-organisms is found in the
oral cavity, whether commensal, facultative pathogenic or obligatory pathogenic. Their mutual ratios and
numbers are considerably affected by probiotic bacteria of the oral microbiota, particularly by their products,
such as bacteriocins. The probiotics most frequently living in the oral cavity include Lactobacillus reuteri,
Streptococcus salivarius and Bacillus coagulans. This study is focused on oral probiotics which could
improve the health of the oral cavity and prevent development of dental carries. Basic techniques, which
are necessary in research of oral microbiota that could be potentially beneficial in dental microbiology, are
also presented. We recommended these techniques based on our experiences in this field. In this study we
describe microbiological methods for obtaining of live bacterial strains in dental plaque or dental calculus,
that are used for preparation of the bacterial strains´ collection and their storage possibilities for next testing.
Study also describes two sensitive molecular methods usable for identification of these bacterial strains, the
first with help of 16S rRNA and next blast n analysis based on consensus DNA sequences, and the second
based on MALDI-TOF mass spectrometry. PCR methods for deeper characterization of selected bacterial
strains e.g. pathogenic like Streptococcus mutans or potentially beneficial bacteria such as Str. salivarius
and Lactobacillus spp. are also described. These methods are based on detection of genes coding production
of bacteriocins or coding genes responsible for pathogenicity e.g. glucosyl or fructosyl transferaze genes.
We also recommend the method for detection of hard cultivable spirochetes based on the morphological
characteristics with help of VFQTOPF method and next by PCR methods used for detection of Treponema
denticola in dental plaque samples.