TY - JOUR
AR - COR-2018-2-101
TI - Inhibitory effect of quercetin on protein expression CtBP1 and migration of hepatocellular carcinoma cell SMMC-7721
AU - Jianhong , Tang
AU - Lihui , Bian
AU - Shengjun , Xiao
AU - Xiaoling , Zhang
AU - Xiaoyu , Wang
AU - Yueli , Yang
JO - Clinical Oncology and Research
PY - 2018
DA - Mon 11, Jun 2018
SN - 2613-4942
DO - http://dx.doi.org/10.31487/j.COR.2018.02.001
UR - https://www.sciencerepository.org/inhibitory-effect-of-quercetin-on-protein-expression-CtBP1-and-migration-of-hepatocellular-carcinoma-cell-SMMC-7721_COR-2-101 
KW - Quercetin, CtBP1, E-cadherin, EMT, Hepatocellular carcinoma
AB - Our study aimed to investigate the potential role of quercetin in the migration of hepatocellular carcinoma
cells, SMMC-7721, and its underlying mechanism. Cell monolayer scratching assay was performed to
explore the role of quercetin in the migration ability of SMMC-7721 cells. Western Blot and
immunofluorescence assay were conducted to detect the protein expression of transcriptional co-suppressor,
CtBP1, and epithelial adhesion molecule, E-cadherin. The migration ability of SMMC-7721 cells was
decreased by the addition of quercetin in a time and concentration dependent manner. Results of western
blot and immunofluorescence assay demonstrated that quercetin inhibited the expression of CtBP1, while
up-regulated the expression of E-cadherin. These findings suggest that quercetin contributed to the upregulation of E-cadherin through inhibition of CtBP1 expression. This in turn hinders the underlying EMT
process, and finally prevents the invasiveness and metastasis of HCC cells. However, the mechanism how
quercetin up-regulation CtBP1 in HCC cells still need to be elucidated.