TY - JOUR
AR - CMR-2020-1-103
TI - Bfp Adhesions and EspA Filament by Enteropathogenic E.Coli in Relation to Pathogenesis of Infantile Diarrhea in Children Under 5 Years in Mansoura University Children Hospital
AU - Ghada, Barakat
AU - Mohamed Elkenawy, 
AU - Shorouq Eletribi, 
JO - Clinical Microbiology and Research
PY - 2020
DA - Mon 13, Apr 2020
SN - 2674-418X
DO - http://dx.doi.org/10.31487/j.CMR.2020.01.03
UR - https://www.sciencerepository.org/bfp-adhesions-and-espa-filament_CMR-2020-1-103 
KW - Bfp adhesions, EspA filament, enteropathogenic E. coli, infantile diarrhea
AB - Background: Our study was applied aiming to detect the biofilm formation by the Enteropathogenic E. coli
causing diarrhea and to investigate the relation between the Bfp adhesions and EspA filament containing
strains with the severity of diarrhea in children under 5 years in Mansoura University Children Hospital,
Egypt.
Methods: It was carried out by identification of EPEC, Serological agglutination test, Biofilm Formation
under static condition at 3,6,9,24 hours.
Results: Out of 103 children with diarrhea, 50 E. coli were isolated, 16 (32%) were EPEC and 34 (68%)
were non-EPEC. The CFU mean± SD of biofilm of 16 EPEC isolates at 4 time points 3, 6, 9, 24h are 108-
1013, 69.5910±24.9310, 106-109, 20.2948±3.96778, 107-1011, 13.92×1010±3.376×1010, 109-1015,
6.402×1013± 2.496×1013. The relation of the biofilm formation and the severity of diarrhea in 16 EPEC
isolates including 5 typical EPEC isolates and11 atypical EPEC reveals significant relation between the
duration of the diarrhea and biofilm formation P=0.001**.
Conclusion: EPEC is capable of forming biofilms on plastic under static and flow through-continuous
conditions which correlate to the severity of the diarrhea in children under 5 years.