Table 3: Tryptophan metabolism pathways balance shifts
following chronic unpredictable stress.
|
|
0 days CUS |
10 days CUS |
20 days CUS |
30 days CUS |
Main effects |
|
Brain metabolite ratios |
|||||
|
5-HT/TRP |
0.07345 (0.0284) |
0.02002 (0.00375) †† |
0.0163 (0.0027†† |
0.02424 (0.00308) † |
*p<0.05 |
|
KYN/TRP |
0.41 (0.0215) |
0.6 (0.0453) †† |
0.52 (0.0494) |
0.96 (0.5) |
n.s. |
|
KYNA/KYN |
0.27 (0.071) |
0.037 (0.0087) †† |
0.079 (0.016) † |
0.19 (0.058) |
**p<0.01 |
|
|
|
|
|
|
|
|
Total oxidative kynurenines (μM) |
1.186 (0.112) |
2.585 (0.352) † |
2.247 (0.367) |
2.072 (0.788) |
n.s. |
|
KAT-dependent kynurenines (μM) |
0.05669 (0.00411) |
0.01825 (0.00307) † |
0.03460 (0.0418) |
0.06787 (0.0185) |
*p<0.05 |
|
Ratio of oxidative KYNs/KATs |
21.26 (2.7) |
151.4 (19.22) †††† |
68.65 (14.78) |
28.88 (4.07) |
****p<0.0001 |
5-HT: Serotonin; TRP: Tryptophan; KYN:
Kynurenine; KYNA: Kynurenic Acid; KAT: Kynurenine Aminotransferase.
Kynurenine
pathway metabolism was analysed by comparing concentrations (μM) to calculate a
ratio value. Total oxidative kynurenines include 3-hydroxykynurenine,
3-hydroxyanthranilic acid, picolinic acid, quinolinic acid. Total KATs include
kynurenic acid and xanthurenic acid.
Ratio
data represent sample means (s.e.m.) n = 4-6 samples per group.
*:
Main effect of CUS, p < 0.05.
†:
Dunnett’s post hoc comparison with 0 days CUS control group.
*,†:
p<0.05; **,††: p<0.01.