Table 1: Levels of salivary analytes and reliability indices between measures taken at rest for UWS, SWS and Bud saliva collection methods for male and female cohorts. Flow rate data was not available for the Bud method. Saliva was collected at two intervals 30 min apart (T1 and T2). Due to the staggered nature of sampling for each method, these intervals are: UWS, 15 and 45 min; SWS, 25 and 55 min; and Bud, 20 and 50 min. A significant % difference between measures is indicated with an asterisk (**, P<0.001; *, P<0.05) and a trend for a difference with a superscript hash (#, 0.1<P>0.05). For PCC measures, a moderate strength relationship (*) is defined as a coefficient ≥0.5 and a strong relationship (**) as a coefficient ≥ 0.7. For LOA and RLOA, error is shown in brackets (+/- and x/÷, respectively).
|
|
Rest T1 |
Rest T2 |
% Difference |
Paired t-test |
r |
CV |
ICC |
SEM |
95% CI |
LOA (Mean bias ±2SD) |
RLOA (Mean bias x/÷2SD) |
|
|
Flow Rate (g·min-1) |
||||||||||||
|
UWS |
Males |
0.64 (0.47) |
0.72 (0.57) |
8.9 |
0.176 |
0.970** |
24.2 |
0.97 |
0.09 |
0.18 |
0.09 ± 0.32 |
1.09 x/÷ 1.70 |
|
Females |
0.34 (0.13) |
0.40 (0.12) |
21.3 |
0.010* |
0.860** |
17.8 |
0.88 |
0.04 |
0.09 |
0.06 ± 0.13 |
1.22 x/÷ 1.90 |
|
|
SWS |
Males |
2.83 (1.91) |
3.02 (1.77) |
6.1 |
0.313 |
0.970** |
16.4 |
0.98 |
0.26 |
0.52 |
0.18 ± 0.94 |
1.06 x/÷ 1.52 |
|
Females |
1.71 (0.48) |
1.48 (0.28) |
12.1 |
0.133 |
0.29 |
30.2 |
0.37 |
0.25 |
0.49 |
-0.23 ± 0.95 |
0.88 x/÷ 1.74 |
|
|
Osmolality (mOsmol·kg-1) |
||||||||||||
|
UWS |
Males |
65.4 (15.8) |
65.9 (18.7) |
0.3 |
0.85 |
0.926** |
11 |
0.96 |
3.38 |
6.77 |
0.50 ± 14.17 |
1.00 x/÷ 1.18 |
|
Females |
57.3 (15.1) |
56.0 (11.8) |
0.2 |
0.65 |
0.788** |
16.4 |
0.87 |
4.52 |
9.03 |
-1.25 ± 18.20 |
0.99 x/÷ 1.35 |
|
|
SWS |
Males |
96.1 (24.7) |
94.8 (24.5) |
1.9 |
0.63 |
0.954** |
8 |
0.98 |
3.68 |
7.36 |
-1.38 ± 14.96 |
0.98 x/÷ 1.18 |
|
Females |
75.8 (12.6) |
75.6 (13.8) |
0.6 |
0.97 |
0.44 |
18.5 |
0.63 |
6.79 |
13.59 |
-0.17 ± 27.37 |
0.99 x/÷ 1.45 |
|
|
Bud |
Males |
76.4 (13.7) |
75.9 (18.1) |
1.4 |
0.91 |
0.753** |
15.6 |
0.86 |
5.63 |
11.27 |
-0.50 ± 23.33 |
0.99 x/÷ 1.34 |
|
|
Females |
72.7 (20.6) |
70.7 (15.4) |
1.8 |
0.65 |
0.728** |
19.7 |
0.83 |
6.85 |
13.7 |
-2.00 ± 27.65 |
0.98 x/÷ 1.39 |
|
Na (mmol·L-1) |
||||||||||||
|
UWS |
Males |
4.53 (2.15) |
5.10 (1.85) |
28.3 |
0.451 |
0.51 |
41.3 |
0.68 |
0.98 |
1.96 |
0.56 ± 3.90 |
1.18 x/÷ 2.22 |
|
Females |
6.37 (3.15) |
5.00 (1.31) |
11 |
0.136 |
0.46 |
49.4 |
0.45 |
1.45 |
2.9 |
-1.37 ± 5.51 |
0.82 x/÷ 2.07 |
|
|
SWS |
Males |
18.6 (11.3) |
19.0 (11.7) |
0.7 |
0.85 |
0.909** |
26.2 |
0.96 |
2.32 |
4.65 |
0.35 ± 9.63 |
0.99 x/÷ 1.64 |
|
Females |
14.8 (6.12) |
13.7 (6.85) |
13.5 |
0.18 |
0.915** |
19.4 |
0.95 |
1.43 |
2.87 |
-1.14 ± 5.42 |
0.86 x/÷ 1.61 |
|
|
Bud |
Males |
13.0 (3.54) |
12.0 (2.92) |
7 |
0.37 |
0.580* |
24.1 |
0.74 |
1.47 |
2.95 |
-1.03 ± 5.90 |
0.93 x/÷ 1.60 |
|
Females |
11.0 (4.23) |
11.0 (4.22) |
0.3 |
0.98 |
0.783** |
25.3 |
0.89 |
1.33 |
2.67 |
-0.02 ± 5.45 |
1.00 x/÷ 1.54 |
|
|
K (mmol·L-1) |
||||||||||||
|
UWS |
Males |
19.7 (4.11) |
21.3 (6.15) |
10.6 |
0.528 |
0.24 |
31.9 |
0.38 |
3.22 |
6.43 |
1.54 ± 12.83 |
1.06 x/÷ 1.73 |
|
Females |
21.9 (5.99) |
19.5 (4.81) |
9.3 |
0.060# |
0.747** |
19.3 |
0.81 |
2.21 |
4.41 |
-2.41 ± 7.82 |
0.89 x/÷ 1.43 |
|
|
SWS |
Males |
21.1 (4.34) |
21.5 (5.44) |
1.1 |
0.39 |
0.992** |
6 |
0.98 |
0.64 |
1.27 |
0.41 ± 2.51 |
1.01 x/÷ 1.10 |
|
Females |
21.7 (3.71) |
21.1 (3.46) |
2.8 |
0.24 |
0.897** |
7.8 |
0.94 |
0.84 |
1.68 |
-0.60 ± 3.26 |
0.97 x/÷ 1.16 |
|
|
Bud |
Males |
19.2 (3.29) |
19.0 (4.35) |
1.8 |
0.81 |
0.865** |
11.7 |
0.92 |
1.06 |
2.11 |
-0.20 ± 4.36 |
0.98 x/÷ 1.20 |
|
Females |
18.9 (3.25) |
18.2 (5.00) |
6 |
0.49 |
0.738** |
18.4 |
0.81 |
1.68 |
3.35 |
-0.74 ± 6.70 |
0.94 x/÷ 1.52 |
|
|
Cl (mmol·L-1) |
||||||||||||
|
UWS |
Males |
32.6 (13.2) |
35.9 (20.4) |
13.8 |
0.634 |
0.47 |
53.8 |
0.62 |
8.94 |
17.88 |
3.24 ± 36.1 |
1.04 x/÷ 2.25 |
|
Females |
49.6 (27.2) |
43.1 (23.3) |
9.7 |
0.193 |
0.806** |
34.9 |
0.88 |
8.36 |
16.72 |
-6.48 ± 31.7 |
0.86 x/÷ 1.76 |
|
|
SWS |
Males |
34.3 (10.9) |
36.6 (15.7) |
3.5 |
0.36 |
0.943** |
18.5 |
0.94 |
3.27 |
6.53 |
2.25 ± 12.9 |
1.04 x/÷ 1.34 |
|
Females |
38.4 (17.0) |
35.4 (13.2) |
7.3 |
0.14 |
0.936** |
17.7 |
0.95 |
3.45 |
6.9 |
-3.03 ± 12.8 |
0.93 x/÷ 1.34 |
|
|
Bud |
Males |
32.3 (13.1) |
30.5 (18.4) |
9.5 |
0.6 |
0.883** |
29.3 |
0.92 |
4.41 |
8.81 |
-1.78 ± 18.0 |
0.90 x/÷ 1.60 |
|
|
Females |
47.3 (27.9) |
45.6 (27.5) |
5.2 |
0.47 |
0.967** |
15.3 |
0.98 |
3.48 |
6.95 |
-1.63 ± 14.0 |
0.95 x/÷ 1.44 |
|
sIgA (mg·L-1) |
||||||||||||
|
UWS |
Males |
65.7 (42.2) |
66.8 (52.0) |
3.5 |
0.895 |
0.900** |
34.9 |
0.94 |
10.86 |
21.72 |
1.12 ± 45.3 |
0.91 x/÷ 2.08 |
|
Females |
86.4 (49.0) |
60.2 (35.8) |
8.3 |
0.12 |
0.21 |
61.9 |
0.56 |
24.06 |
48.12 |
-26.2 ± 88.9 |
0.71 x/÷ 5.05 |
|
|
SWS |
Males |
29.8 (15.5) |
31.0 (23.3) |
10.3 |
0.71 |
0.960** |
31 |
0.95 |
4.46 |
8.92 |
1.27 ± 18.5 |
0.89 x/÷ 1.96 |
|
Females |
28.3 (17.5) |
22.8 (8.8) |
14.9 |
0.24 |
0.709** |
50.4 |
0.71 |
6.59 |
13.18 |
-5.49 ± 25.2 |
0.85 x/÷ 2.06 |
|
|
Bud |
Males |
34.6 (18.7) |
36.1 (23.5) |
1 |
0.79 |
0.772** |
42.3 |
0.87 |
7.08 |
14.16 |
1.45 ± 29.3 |
1.01 x/÷ 2.15 |
|
Females |
31.2 (28.7) |
29.0 (13.5) |
15.5 |
0.76 |
0.745** |
68.7 |
0.75 |
9.93 |
19.86 |
-2.21 ± 40.5 |
1.17 x/÷ 2.95 |
|
|
α-amylase activity (U·mL-1) |
||||||||||||
|
UWS |
Males |
34.6 (20.4) |
38.2 (22.1) |
12.3 |
0.050* |
0.983** |
11.7 |
0.98 |
26.75 |
53.51 |
36.4 ± 83.2 |
1.11 x/÷ 1.25 |
|
Females |
25.7 (23.5) |
29.5 (24.9) |
22.4 |
0.030* |
0.983** |
15.6 |
0.99 |
26.71 |
53.43 |
35.8 ± 83.8 |
1.18 x/÷ 1.46 |
|
|
SWS |
Males |
49.9 (18.3) |
48.7 (18.3) |
2.8 |
0.76 |
0.821** |
22.2 |
0.91 |
51.75 |
103.49 |
-12.4 ± 214.4 |
0.97 x/÷ 1.48 |
|
Females |
37.4 (20.5) |
39.3 (22.0) |
4.6 |
0.24 |
0.971** |
13.2 |
0.98 |
25.99 |
51.99 |
18.9 ± 96.0 |
1.05 x/÷ 1.35 |
|
|
Bud |
Males |
38.2 (15.9) |
40.7 (17.9) |
4.6 |
0.54 |
0.798** |
27.6 |
0.89 |
52.55 |
52.55 |
24.8 ± 213.1 |
1.05 x/÷ 1.82 |
|
Females |
32.6 (25.3) |
28.5 (23.3) |
7.4 |
0.15 |
0.931** |
28.3 |
0.96 |
45.12 |
90.24 |
-39.5 ± 67.4 |
0.92 x/÷ 1.96 |
|
|
Cortisol (nmol·L-1) |
||||||||||||
|
UWS |
Males |
4.21 (0.73) |
4.02 (0.48) |
3.7 |
0.21 |
0.893** |
8.9 |
0.89 |
0.19 |
0.39 |
-0.20 ± 0.72 |
0.96 x/÷ 1.18 |
|
Females |
5.95 (2.26) |
5.26 (2.16) |
11.4 |
0.003* |
0.960** |
11.3 |
0.96 |
0.45 |
0.9 |
-0.68 ± 1.24 |
0.88 x/÷ 1.20 |
|
|
SWS |
Males |
5.27 (2.87) |
5.43 (4.19) |
4.1 |
0.76 |
0.991** |
26.1 |
0.97 |
0.66 |
1.32 |
0.16 ± 2.74 |
0.96 x/÷ 1.40 |
|
Females |
6.33 (2.40) |
5.16 (1.73) |
20.2 |
0.030* |
0.749** |
27.7 |
0.77 |
0.93 |
1.85 |
-1.17 ± 3.12 |
0.79 x/÷ 1.85 |
|
|
Bud |
Males |
5.21 (2.69) |
4.80 (1.63) |
4.3 |
0.35 |
0.974** |
23.4 |
0.93 |
0.58 |
1.17 |
-0.41 ± 2.29 |
0.96 x/÷ 1.33 |
|
|
Females |
4.80 (2.18) |
4.47 (1.76) |
4.2 |
0.18 |
0.955** |
15.5 |
0.96 |
0.38 |
0.76 |
-0.33 ± 1.41 |
0.96 x/÷ 1.39 |
PCC: Pearson’s Correlation Coefficient; CV: coefficient of variation; ICC: Intra-class Correlation Coefficient; SEM: Standard Error of the Mean; 95% CI: 95% Confidence Interval; LOA: Limits of Agreement; RLOA: Ratio Limits of Agreement.