Table 1: Assay parameters and validation results obtained by applying Isoabsorptive assay spectrophotometric method.
|
Mixture |
PAR & ORP |
|
|
Method Parameters |
ORP |
PAR |
|
Wavelength (nm) |
211 |
280 |
|
Linearity range (µg/mL) (n=3) |
5-50 |
4-22 |
|
Intercept |
0.0427 |
0.0070 |
|
Slope |
0.0485 |
0.0153 |
|
Correlation coefficient (r) |
0.9994 |
0.9996 |
|
Accuracy (Mean ± SD) |
101.41 ± 0.59 |
100.36 ± 1.08 |
|
Precision (±%RSD) |
||
|
Repeatability |
101.81 ± 0.73 |
99.78 ± 1.14 |
|
Intermediate precision |
99.73 ± 0.88 |
99.55 ± 0.77 |
|
Specificity (Mean ± SD) |
100.85 ± 1.20 |
100.17 ± 1.02 |