Table 3: Dataset of 200 interphase nuclei scored (signal pattern) in 11 known normal control peripheral blood specimens for BCR/ABL1 (manufacturer 1) FISH probe.
|
Sample |
Total cells |
Normal Signal pattern |
Abnormal Signal Patterns |
|||||||||||||
|
Patterns |
2R2G |
2R1G |
1R2G |
1F1R1G |
others |
|||||||||||
|
Observers |
O1 |
O2 |
% |
O1 |
O2 |
% |
O1 |
O2 |
% |
O1 |
O2 |
% |
O1 |
O2 |
% |
|
|
1. |
200 |
95 |
96 |
95.5 |
3 |
2 |
2.5 |
2 |
2 |
2 |
0 |
0 |
0 |
0 |
0 |
0 |
|
2. |
200 |
89 |
86 |
87.5 |
3 |
3 |
3 |
4 |
6 |
5 |
4 |
3 |
3.5 |
2 |
0 |
1 |
|
3. |
200 |
88 |
87 |
87.5 |
3 |
6 |
4.5 |
4 |
5 |
4.5 |
5 |
2 |
3.5 |
0 |
0 |
0 |
|
4. |
200 |
92 |
88 |
90 |
4 |
7 |
5.5 |
1 |
0 |
0.5 |
3 |
2 |
2.5 |
3 |
0 |
1.5 |
|
5. |
200 |
91 |
85 |
88 |
4 |
7 |
5.5 |
3 |
3 |
3 |
2 |
5 |
3.5 |
0 |
0 |
0 |
|
6. |
200 |
93 |
87 |
90 |
4 |
3 |
3.5 |
2 |
6 |
4 |
1 |
1 |
1 |
3 |
0 |
1.5 |
|
7. |
200 |
89 |
84 |
86.5 |
4 |
8 |
6 |
5 |
2 |
3.5 |
2 |
6 |
4 |
0 |
0 |
0 |
|
8. |
200 |
88 |
83 |
85.5 |
8 |
14 |
11 |
1 |
0 |
0.5 |
3 |
3 |
3 |
0 |
0 |
0 |
|
9. |
200 |
92 |
94 |
93 |
5 |
3 |
4 |
0 |
0 |
0 |
3 |
3 |
3 |
0 |
0 |
0 |
|
10. |
200 |
92 |
91 |
91.5 |
0 |
2 |
1 |
6 |
7 |
6.5 |
2 |
0 |
1 |
0 |
0 |
0 |
|
11. |
200 |
90 |
89 |
89.5 |
3 |
5 |
4 |
5 |
0 |
2.5 |
2 |
2 |
2 |
4 |
0 |
2 |
|
MEAN |
|
90.8 |
88.1 |
89.5 |
3.7 |
5.4 |
4.5 |
3 |
2.8 |
2.9 |
2.4 |
2.4 |
2.4 |
1.0 |
0 |
0.5 |
|
SD |
|
2.1 |
3.8 |
2.82 |
1.8 |
3.3 |
2.4 |
1.8 |
2.6 |
1.9 |
1.3 |
1.7 |
1.2 |
1.5 |
0 |
0.7 |
|
BETAINV (%) |
|
|
|
|
|
|
13.8 |
|
|
9.5 |
|
|
8.4 |
|
|
4.4 |
O: observer, 2R2G: 2 red, 2 green; 2R1G: 2 red, 1 green; 1R2G: 1 red, 2 green;
Note: 1F1R1G: 1 fusion 1 red 1 green; others: less common signals. SD: Standard deviation.