Table 2: Accuracy, precision, sensitivity and specificity of the BCR/ABL1 probe (manufacturer one).

ACCURACY

No. of samples

Scored (negative control)

No. of samples with probes bound to its own locus

No. of probes bound unspecifically

4

4

0

PRECISION

Pattern

(negative control)

O1

(100 nuclei)

O2

(100 nuclei)

SD

Precise

(Mean±SD)

2R2G

90.8

88.1

2.82

Y

2R1G

3.7

5.4

2.4

Y

1R2G

3

2.8

1.9

Y

1F1R1G

2.4

2.4

1.2

Y

SENSITIVITY

Cases

No. Metaphases

scored (positive controls)

BCR/ABL1 Positive metaphases

BCR/ABL1 Negative metaphases

Sensitivity %

 

 

1

5

5

0

100%

 

2

5

5

0

 

3

5

5

0

 

4

5

5

0

 

SPECIFICITY

 

Cases

No. of metaphases scored (negative control)

BCR/ABL1 positive metaphases

BCR/ABL1 negative metaphases

Specificity %

 

1

5

0

5

100%

 

2

5

0

5

 

3

5

0

5

 

4

5

0

5

 

O1: Observer1; O2: Observer 2, R: red, G: green; F: fusion; Y: precise.