Table 2: Accuracy, precision, sensitivity and specificity of the BCR/ABL1 probe (manufacturer one).
|
ACCURACY |
|||||||||
|
No. of samples Scored (negative control) |
No. of samples with probes bound to its own locus |
No. of probes bound unspecifically |
|||||||
|
4 |
4 |
0 |
|||||||
|
PRECISION |
|||||||||
|
Pattern (negative control) |
O1 (100 nuclei) |
O2 (100 nuclei) |
SD |
Precise (Mean±SD) |
|||||
|
2R2G |
90.8 |
88.1 |
2.82 |
Y |
|||||
|
2R1G |
3.7 |
5.4 |
2.4 |
Y |
|||||
|
1R2G |
3 |
2.8 |
1.9 |
Y |
|||||
|
1F1R1G |
2.4 |
2.4 |
1.2 |
Y |
|||||
|
SENSITIVITY |
|||||||||
|
Cases |
No. Metaphases scored (positive controls) |
BCR/ABL1 Positive metaphases |
BCR/ABL1 Negative metaphases |
Sensitivity %
|
|
||||
|
1 |
5 |
5 |
0 |
100% |
|
||||
|
2 |
5 |
5 |
0 |
|
|||||
|
3 |
5 |
5 |
0 |
|
|||||
|
4 |
5 |
5 |
0 |
|
|||||
|
SPECIFICITY |
|
||||||||
|
Cases |
No. of metaphases scored (negative control) |
BCR/ABL1 positive metaphases |
BCR/ABL1 negative metaphases |
Specificity % |
|
||||
|
1 |
5 |
0 |
5 |
100% |
|
||||
|
2 |
5 |
0 |
5 |
|
|||||
|
3 |
5 |
0 |
5 |
|
|||||
|
4 |
5 |
0 |
5 |
|
|||||
O1: Observer1; O2: Observer 2, R: red, G: green; F: fusion; Y: precise.