Table 2: Accuracy, precision, sensitivity and specificity of the BCR/ABL1 probe (manufacturer one).
ACCURACY |
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No. of samples Scored (negative control) |
No. of samples with probes bound to its own locus |
No. of probes bound unspecifically |
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4 |
4 |
0 |
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PRECISION |
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Pattern (negative control) |
O1 (100 nuclei) |
O2 (100 nuclei) |
SD |
Precise (Mean±SD) |
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2R2G |
90.8 |
88.1 |
2.82 |
Y |
|||||
2R1G |
3.7 |
5.4 |
2.4 |
Y |
|||||
1R2G |
3 |
2.8 |
1.9 |
Y |
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1F1R1G |
2.4 |
2.4 |
1.2 |
Y |
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SENSITIVITY |
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Cases |
No. Metaphases scored (positive controls) |
BCR/ABL1 Positive metaphases |
BCR/ABL1 Negative metaphases |
Sensitivity %
|
|
||||
1 |
5 |
5 |
0 |
100% |
|
||||
2 |
5 |
5 |
0 |
|
|||||
3 |
5 |
5 |
0 |
|
|||||
4 |
5 |
5 |
0 |
|
|||||
SPECIFICITY |
|
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Cases |
No. of metaphases scored (negative control) |
BCR/ABL1 positive metaphases |
BCR/ABL1 negative metaphases |
Specificity % |
|
||||
1 |
5 |
0 |
5 |
100% |
|
||||
2 |
5 |
0 |
5 |
|
|||||
3 |
5 |
0 |
5 |
|
|||||
4 |
5 |
0 |
5 |
|
O1: Observer1; O2: Observer 2, R: red, G: green; F: fusion; Y: precise.