Table 1: Antibodies used for IHC staining and their characteristics.
|
1º Ab |
Ig type |
Concentration |
Antigen retrieval |
Serum |
2 º Ab |
|
Anti α-smooth muscle actin (mouse monoclonal) (Abcam) |
IgG2a
|
2 µg/mL |
0.1% Triton-100, 20 min |
goat |
Goat anti-mouse IgG H&L (Biotin) |
|
Anti-MUC 1 (rabbit monoclonal) (Abcam) |
IgG
|
1.53µg/mL |
0.1% Triton-100, 20 min |
horse |
Biotinylated anti-rabbit IgG |
|
Isotypic control (goat anti-mouse) (Abcam) |
IgG2a |
1.1 µg/mL
|
0.1% Triton-100, 20 min
|
horse |
Biotinylated anti-rabbit IgG |
|
Isotypic control (goat anti-mouse) (Abcam) |
IgG |
1.53 µg/mL |
0.1% Triton-100, 20 min |
horse |
Biotinylated anti-rabbit IgG |
|
Anti-cytokeratin 5 [K5] (rabbit monoclonal) (Abcam) |
IgG |
1 µg/mL |
Citrated buffer x1, heated for 20min |
horse |
Biotinylated anti-rabbit IgG |
|
Anti-cytokeratin 19 [CK19] (mouse monoclonal) (Abcam) |
IgG2a |
1 µg/mL |
Citrated buffer x1, heated for 20min |
horse |
Biotinylated anti-mouse IgG |