Table 2: Proportion of amplified cells with MYCN amplification in every patient. MYCN amplification was quantified in every cell as significant, discreet and without amplification (the percentage given without the brackets is the number of analysed cells in the bone marrow, while the number given in the brackets represents proportion of cells in the tumor tissue).

Presence of amplification

 

 

Patient 1

Patient 2

Patient 3

Patient 4

Patient 5

before therapy

after 6 months

before therapy

after 6 months

before therapy

after 6 months

A. No MYCN amplification

 

 

13%  (9%)

15%

17%

(36%)

21%

83%

(77%)

97%

(80%)

100%

(100%)

B.MYCN gain

 

 

/

 

/

(10%)

5%

7%

(11%)

3%

(20% )

3 copies

(trisomy 2)

/

C.MYCN amplification

C1. discrete

 

22%

(28%)

28%

11%

(28%)

9%

6%

(7%)

/

/

/

C2.significant

2a

60%

(51%)

45%

59%

(20%)

42%

4%

(5%)

/

/

/

2b

5%

(12%)

12%

13%

(6%)

23%

0%

(0%)

/