Table 1: HPLC parameters
|
Time (min) |
Reagent Aa (%) |
Reagent Bb (%) |
Flow (mL/min) |
|
0.0 |
96 |
4 |
0.6 |
|
13.0 |
93 |
7 |
0.6 |
|
14.0 |
70 |
30 |
0.6 |
|
15.0 |
70 |
30 |
0.6 |
|
15.1 |
96 |
4 |
0.6 |
|
16.0 |
96 |
4 |
0.7 |
a citrate buffer pH 4.5; b acetonitrile