Table 2: Acronym, net charge, predictive index of helicity (% AGADIR) and bacterial inhibition.

Acronym

Net Charge

Index of helicity

(% AGADIR)

Bacterial inhibition

*MIC90

FK16

4

1,9

6,25μM

Tat-FK16

10

1,85

6,25μM

IV13

2

0,89

>100μM

Tat-IV13

10

1,85

3,125μM

Tat-rev IV13

10

1,43

6,25μM

For Net Charge calculation see. An algorithm based on helix-coil transition theory, AGADIR, was used to predict helical propensity [16].

*The MIC (μM) of each peptide is an average of triplicate measurements performed by a dilution method in 96-well polypropylene microplate. The MICs90 was considered to be the peptide concentration that inhibited growth of 90% of the tested strains.