Table 2: Acronym, net charge, predictive index of helicity (% AGADIR) and bacterial inhibition.
Acronym |
Net Charge |
Index of helicity (% AGADIR) |
Bacterial inhibition *MIC90 |
FK16 |
4 |
1,9 |
6,25μM |
Tat-FK16 |
10 |
1,85 |
6,25μM |
IV13 |
2 |
0,89 |
>100μM |
Tat-IV13 |
10 |
1,85 |
3,125μM |
Tat-rev IV13 |
10 |
1,43 |
6,25μM |
For Net Charge calculation see. An algorithm based on helix-coil transition theory, AGADIR, was used to predict helical propensity [16].
*The MIC (μM) of each peptide is an average of triplicate measurements performed by a dilution method in 96-well polypropylene microplate. The MICs90 was considered to be the peptide concentration that inhibited growth of 90% of the tested strains.