Table 1: Oligonucleotide sequences of the primers designed for miRNA measurement.
Oligonucleotide name |
Product name |
Sequence (5′—3′) |
Product by |
hsa-miR-155-5p |
Forward primer |
CCGTTAATGCTAATCGTGA |
Macrogen |
Reverse primer |
GAGCAGGGTCCGAGGT |
||
probe |
FAM-AGGGGTGTCGTATGCAGTGC-BHQ1 |
||
RT |
GTCGTATGCAGAGCAGGGTCCGAGGTATTCGCACTGCATACGACACCCCT |
||
MiRCURY LAN Inhibitor |
CCTATCACGATTAGCATT |
Exiqon |
|
hsa-miR-146a-5p |
Forward primer |
CCGATGAGAACTGAATTCC |
Macrogen |
Reverse primer |
GAGCAGGGTCCGAGGT |
||
probe |
FAM -TGGGTTGTCGTATGCAGTGC-BHQ1 |
||
RT |
GTCGTATGCAGAGCAGGGTCCGAGGTATTCGCACTGCATACGACAACCCA |
||
MiRCURY LAN Inhibitor |
ACCCATGGAATTCAGTTCTC |
Exiqon |
|
has-SNORD-47 |
Forward primer |
ATCACTGTAAAACCGTTCCA |
Macrogen |
Reverse primer |
GAGCAGGGTCCGAGGT |
||
probe |
FAM-TGATTCTGAGGTTGTCGTATGCA-BHQ1 |
||
RT |
GTCGTATGCAGAGCAGGGTCCGAGGTATTCGCACTGCATACGACAACCTC |
||
Scramble siRNA |
Scramble siRNA |
GTGTAACACGTCTATACGCCCA |
Exiqon |