Table 1: Chromatographic conditions for the proposed method.
|
Parameters |
Conditions |
|
Column |
RP BDS Hypersil® C18 (150 x 4.60 mm, 5μm) (phenomenex, USA) |
|
Mobile phase |
Acetonitrile : 0.05 M phosphate buffer pH 3.50 (55 : 45) |
|
UV detection, nm |
254 |
|
Flow rate, mL/min |
1.50 |
|
Injected volume, µl |
20 |
|
Temperature |
40 |