Hemorrhage Hypotension Influences on Plasma Glucose Concentration: A New Approach for Evaluating Baroreflex Effects on Metabolic Adjustments

Author Info

Daniel Carvalho de Lima Helton Oliveira Campos Lucas Rios Drummond Simonton Andrade Silveira Andrea Siqueira Haibara Cândido Celso Coimbra

Corresponding Author

Cândido Celso Coimbra
Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

A B S T R A C T

The present study shows that hyperglycemic response to hemorrhage is an easy method for evaluating metabolic adjustments in normal rats and in other models that show autonomic and metabolic imbalances. In this regard, the baroreflex control of hyperglycemic response induced by hemorrhage hypotension was evaluated by removing blood through the jugular catheter (1.2 mL/100g b.w./ 2 min). Blood samples (0.2mL) were collected immediately before hemorrhage and at 5, 10, 20 and 30 minutes after hemorrhage [1, 2]. In addition, the baroreflex control of heart rate was assessed by pharmacological test, using intravenous doses of phenylephrine hydrochloride (PE, 10 μg/mL) and sodium nitroprusside (SNP, 10 μg/mL) in random order. Heart rate and blood pressure were measured through the insertion of a polyethylene catheter inserted into the abdominal aorta through the left femoral artery. The derived variables of baroreflex control to heart rate and hemorrhage hyperglycemia response were measured according to the following equation: Y = A1/{1+ exp[A2(X - A3)]} + A4. The evaluation of a new approach for studying the baroreflex effects on metabolic adjustments was done with animals fed with a hypercaloric diet. Hypercaloric diet induced an upward shift in the baroreflex curve to heart rate (p<0.05) and an increased heart rate reflex due to the change in MAP during the operating range (p<0.05). It also shifted the baroreflex curve to a higher level of hyperglycemic response to hemorrhage (p<0.05), as well as, increased maximal gain (p<0.05) and augmented hyperglycemic response to hemorrhage hypotension during the operating range (p<0.05). Therefore, we propose that the baroreflex control of hyperglycemic response should be a useful tool for evaluating metabolic dysfunction related to hemorrhage in models of animals that show autonomic imbalance, such as malnutrition, hypertension, diabetes and obesity.

Article Info

Article Type

Research Article

Publication history

Received: Thu 16, Jun 2022
Accepted: Fri 01, Jul 2022
Published: Mon 18, Jul 2022

Copyright

© 2023 Cândido Celso Coimbra. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.JICOA.2022.02.03