Histomorphometric and Radiographic Analysis of Biological Responses Following the Use of Pure Hydroxyapatite and Hydroxyapatite with Collagen

Histomorphometric and Radiographic Analysis of Biological Responses Following the Use of Pure Hydroxyapatite and Hydroxyapatite with Collagen

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Juliana Larocca de Geus
Department of Endodontics, Paulo Picanço School of Dentistry, Fortaleza, Ceará, Brazil

A B S T R A C T

Background and Objectives: The objective of this study was to evaluate in vivo two new biomaterials for bone substitution aiming to determining their ability to enable bone neoformation in critical-size defects in rats’ calvaria. Methods: Synthetic hydroxyapatite were developed – Pure hydroxyapatite (HaP) and Hydroxyapatite with collagen (HCol). The third synthetic hydroxyapatite used as a comparing element was the commercial hydroxyapatite Alobone® (HaAl). Sixty Wistar rats divided randomly into four groups (Group 1, HaP; Group 2, HaCol; Group 3, HaAl and Group 4, Control). Critical size defects of 8mm were performed in the calvaria, and the three biomaterials were implanted. All groups also received a collagen membrane. Results: On day 30, the inflammatory response on the defect was practically absent in all groups, mainly when compared to the period of 7 days. Some areas of bone neoformation were seen. There was predominance of osteoblasts, osteocytes, and macrophages in lower amounts. Regarding the radiographic aspect, after 30 days, a marked biodegradation of the hydroxyapatite and signs of bone neoformation were observed. Conclusions: It was concluded that the results of our study in vivo can be used as a preliminary source of information about biocompatibility, biodegradability, and bone neoformation from the implant of new biomaterials in vivo.

Article Info

Article Type
Research Article
Publication history
Received: Tue 10, Mar 2020
Accepted: Tue 24, Mar 2020
Published: Tue 31, Mar 2020
Copyright
© 2023 Juliana Larocca de Geus. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.DOBCR.2020.01.09