Long-term dental adhesive toxicity on human gingival fibroblasts and epithelial cells

Long-term dental adhesive toxicity on human gingival fibroblasts and epithelial cells

Author Info

Corresponding Author
Babu J.P
Department of Bioscience Research Center, and Craniofacial Research center, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, USA

A B S T R A C T

Dentin adhesive system (DAS) components released can induce cytotoxic effects. We hypothesize that media obtained from long-term stored DAS are cytotoxic, depending on the DBS type/composition and modulation of cytotoxicity is time dependent. 0.2 mL DMEM media obtained from incubating DAS discs were filter-sterilized, added to the cultured cells (human gingival fibroblast and epithelial cells) for 24 hours and harvested up to 50 days. Cell toxicity was assessed using MTT cell viability assay and LIVE/DEAD backlight staining. PB was cytotoxic to both cell types only from 1h to 3 days. SE was non-toxic up to 1h, but it was up to 50th day, when the toxicity ceased. The SB was slightly more toxic than Scotch, but by day 45 no significant toxicity to the cells was observed. We concluded that all DAS tested were toxic for the cells analyzed, and cytotoxicity was composition/time dependent.

Article Info

Article Type
Research Article
Publication history
Received: Wed 17, Apr 2019
Accepted: Tue 30, Apr 2019
Published: Wed 12, Jun 2019
Copyright
© 2023 Babu J.P. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.DOBCR.2019.03.01