Combination of Ruthenium Dendrimers and Acoustically Propelled Gold Nanowires as a Platform for Active Intracellular Drug Delivery Towards Breast Cancer Therapy
Combination of Ruthenium Dendrimers and Acoustically Propelled Gold Nanowires as a Platform for Active Intracellular Drug Delivery Towards Breast Cancer Therapy
Author Info
Berta Esteban-Fernández de Ávila Hua Gong Joseph Wang Natalia Sanz del Olmo Paula Ortega Francisco Javier de la Mata Gulcin Bolat Sylwia Michlewska Tibor Hianik Zuzana Garaiová
Corresponding Author
Tibor HianikFaculty of Mathematics, Physics and Informatics, Comenius University, Bratislava, Slovakia
A B S T R A C T
In this work, a new class of fluorescently labeled metallodendrimers based on ruthenium and possessing anticancer activity (FITC-CRD13) is combined with graphene oxide modified gold nanowires (GOAuNWs). The resulting complexes were tested as active intracellular transporters being propelled by ultrasound field (US) and using breast cancer cells as a model. Energy dispersive X-ray spectroscopy analysis confirmed the successful modification of GO-AuNWs by dendrimers as shown by the uniform presence of ruthenium over the nanomotor structure corresponding to the ruthenium groups of FITC-CRD13. The binding of dendrimers to the surface of GO-AuNWs was accompanied by quenching their fluorescence signal. Upon the application of an ultrasound field (5 min, 2 V, 2.66 MHz), the complexes were propelled towards MCF7 breast cancer cells, detaching from the GO-nanomotor surface and thus recovering the dendrimer fluorescence signal. Fluorescence signal from US-treated samples was ~1.8 fold higher compared to passive controls. The results obtained in this work suggest that US-propelled AuNWs lead to faster cell internalization, hence accelerating the delivery of the carbosilane ruthenium dendrimers (CRD) payload inside MCF7 cells.
Article Info
Article Type
Short CommunicationPublication history
Received: Tue 20, Aug 2019Accepted: Fri 13, Sep 2019
Published: Wed 20, Nov 2019
Copyright
© 2023 Tibor Hianik. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.DOI: 10.31487/j.COR.2019.04.08