Black Tea Extract prevents 4-nitroquinoline 1-oxide induced oral tumorigenesis in mice by targeting Protein Tyrosine Kinases and associated biological response

Black Tea Extract prevents 4-nitroquinoline 1-oxide induced oral tumorigenesis in mice by targeting Protein Tyrosine Kinases and associated biological response

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Corresponding Author
Madhumita Roy
Department of Environmental Carcinogenesis & Toxicology, Chittaranjan National Cancer Institute, 37, S. P. Mukherjee Road, Kolkata-700 026, India

A B S T R A C T

Oral squamous cell carcinoma of the tongue is the most common type of cancer, common site being buccal mucosa and gingiva. Despite advancement in treatment protocol, recurrence of the disease limits outcome of oral cancer; contributing factors for poor prognosis are activation, overexpression, dysregulation or mutation of many target proteins including protein tyrosine kinases (PTKs). Therefore, it is time to develop preventive measures against oral carcinogenesis, particularly by regulating tyrosine kinases. Natural compounds find a place in cancer prevention; hence, emphasis has been given to investigate the chemopreventive efficacy of black tea extract in 4-nitroquinoline-1-oxide (4NQO)-induced tongue tumorigenesis in C57BL/6 mice. The histological diagnosis of squamous neoplasia was observed at different time intervals in mice treated with 4NQO and tea group, alone or in conjunction. Results indicate the preventive role of black tea in 4NQO-induced lesions. Expressions of different tyrosine kinases, their phosphorylated forms (EGFR, JAK-1, JAK-2, p38 MAPK) and their downstream signalling proteins (PI3K, Akt, Raf1 and Stat3) were determined by western blot analysis at different stages of oral carcinogenesis. 4NQO-induced endogenous ROS generation level and corresponding DNA damage by comet assay were followed in presence and absence of tea. An upward trend of ROS level as well as DNA damage was observed with progression of oral carcinogenesis. Administration of black tea extract inhibited tongue tumorigenesis by down-regulation of protein tyrosine kinases, reducing ROS level and counteracting DNA damage. Black tea therefore exhibited efficient chemo-preventive activity in oral carcinogenesis.

Article Info

Article Type
Research Article
Publication history
Received: Tue 15, Jan 2019
Accepted: Fri 08, Feb 2019
Published: Wed 13, Mar 2019
Copyright
© 2023 Madhumita Roy. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.COR.2019.01.102